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GDF15 抗体 (C-Term) (HRP)

This anti-GDF15 antibody (ABIN1043915) is a Rabbit Polyclonal antibody detecting GDF15 in WB, ELISA, IHC. Suitable for Human, Mouse.
Rockland
产品编号 ABIN1043915
Supplier Product No.: 600-403-b07
发货至: 中国

Quick Overview for GDF15 抗体 (C-Term) (HRP) (ABIN1043915)

抗原

See all GDF15 抗体
GDF15 (Growth Differentiation Factor 15 (GDF15))

适用

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多克隆

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This GDF15 antibody is conjugated to HRP

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
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    C-Term

    Supplier Product No.

    600-403-b07

    Supplier

    Rockland

    原理

    GDF15 Antibody Peroxidase Conjugated

    交叉反应 (详细)

    This antibody reacts with the C-terminus of endogenous NAG-1 protein from human and mouse tissues.

    产品特性

    Synonyms: rabbit anti-NAG1 antibody peroxidase conjugation, HRP conjugated rabbit anti-NAG1 antibody, NAG 1, NAG-1, GDF15, MIC1, MIC-1, GDF-15, PLAB, PTGFB, nonsteroidal anti-inflammatory drug-activated gene, NSAID-activated gene 1 protein, growth differentiation factor 15, macrophage inhibitory compound 1, Placental bone morphogenetic protein, Prostate differentiation factor

    纯化方法

    Anti-NAG-1 (C-terminal specific) was affinity purified from monospecific antiserum by immunoaffinity chromatography.

    免疫原

    Immunogen: Anti-NAG-1 (C-terminal specific) antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminal end of human NAG-1 protein.  A residue of cysteine was added to facilitate coupling to KLH.

    Immunogen Type: Conjugated Peptide

    亚型

    IgG
  • 应用备注

    Immunohistochemistry Dilution: 1:500-1:2,500

    Application Note: Anti-NAG1/GDF15 (C-terminal specific) antibody has been tested by ELISA and is suitable for IHC and western blotting of human and mouse NAG-1 protein.  For detection of NAG-1 in human serum, a sandwich ELISA is suggested using this antibody in combination with anti-NAG-1/GDF15 (N-terminal), H variant or D variant specific antibodies.  Specific conditions for reactivity should be optimized by the end user.  Expect bands in Western blots of approximately 14 and 28 kDa in size corresponding to NAG-1 monomer and dimer, respectively, using the appropriate cell lysate or extract.

    Western Blot Dilution: 1:1,000-1:5,000

    ELISA Dilution: 1:10,000-1:50,000

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度

    1.07 mg/ml

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

    储存液

    Gentamicin sulfate

    注意事项

    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

    有效期

    12 months
  • Baek, Eling: "Changes in gene expression contribute to cancer prevention by COX inhibitors." in: Progress in lipid research, Vol. 45, Issue 1, pp. 1-16, (2006) (PubMed).

  • 抗原

    GDF15 (Growth Differentiation Factor 15 (GDF15))

    别名

    GDF15

    背景

    Background: Non-steroidal anti-inflammatory drug (NSAID) activated gene (NAG-1) is a member of the transforming growth factor-beta (TGF-beta) superfamily. NAG-1 is also known as Macrophage Inhibitory Cytokine-1 (MIC-1), Growth Differentiation Factor 15 (GDF15), Placental Bone Morphogenetic Protein (PLAB), or Prostate Derived Factor (PDF). NAG-1 is expressed in human placenta, prostate and colon. It possesses antitumorigenic and proapoptotic activities. NAG-1 expression is dramatically increased in inflammation, injury and malignancy. Increase of NAG-1 expression is a feature of many cancers including breast, colon, pancreas and prostate. In a number of studies, NAG-1 expression was increased by a number of NSAIDs. This increase in expression may correlate with the chemopreventive effect NSAIDs seem to have with certain cancers. NAG-1 expression is also induced by PPAR gamma ligands and by several dietary compounds such as conjugated linoleic acids (CLAs), naturally occurring fatty acids in ruminant food products, indoles, epicatechin gallate, and genistein. Induced expression of NAG-1 results in stimulation of apoptosis and inhibition of cell growth. Inhibition of NAG-1 induced expression by small interference RNA (siRNA) results in repression of induced apoptosis. NAG-1 expression is regulated by a numbers of transcription factors such as ERG-1 and Sp1. EGR-1 may be necessary for NSAID-induced NAG-1 expression. The study of expression of NAG-1 proteins, including variants, is important to define their potential role as serum biomarkers for cancer diagnosis, treatment monitoring, epidemiology study, and nutrition surveys.

    基因ID

    9518

    UniProt

    Q99988

    途径

    SARS-CoV-2 Protein Interactome
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