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SMAD3 抗体 (Internal Region)

Name: SMAD3 抗体 (Internal Region)
SKU: ABIN1043911
Availability: OutOfStock

Rockland 600-401-e70 SMAD3 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated

Western Blot of Rabbit Anti-SMAD3 antibody. (SMAD3 抗体 (Internal Region))

ELISA

3 images

(1 reference)

产品编号 ABIN1043911

Supplier Product No.: 600-401-e70

发货至: 中国

Datasheet as PDF

抗原 See all SMAD3 抗体

SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))
抗原表位
38

33

27

27

25

12

11

8

6

5

5

5

4

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Internal Region
适用
231

153

135

27

9

9

8

7

7

4

3

3

2

2

1

1
人
宿主
220

24

1
兔
Compatible Secondaries
克隆类型
205

40
多克隆
标记
123

13

12

11

8

6

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

1

1

1

1
This SMAD3 antibody is un-conjugated
应用范围
194

98

76

73

55

53

49

48

28

13

13

8

5

4

3

1
Western Blotting (WB), ELISA

Supplier Product No.

600-401-e70

Supplier

Rockland

原理

SMAD3 Antibody

交叉反应（详细）

SMAD3 Antibody is directed against human Smad3 protein.

产品特性

Synonyms: rabbit anti-SMAD3 antibody, SMAD-3, SMAD 3, mothers against decapentaplegic homolog 3 antibody, MAD homolog 3, Mothers against DPP homolog 3, SMAD family member 3, MADH3, MADH 3, JV15-2

纯化方法

The product was affinity purified from monospecific antiserum by immunoaffinity purification.

过滤

Sterile filtered

免疫原

Immunogen: SMAD3 Antibody was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of human Smad3 protein surrounding amino acid residue 179.
Immunogen Type: Conjugated Peptide

亚型

IgG

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anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 231-425) antibody
SMAD3 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (C-Term) antibody
SMAD3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) antibody
KO Validated SMAD3 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated

应用备注

Application Note: SMAD3 Antibody has been tested for use in ELISA and by western blot. This antibody is suitable in immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48.1 kDa in size corresponding to human Smad3 protein by western blotting in the appropriate tissue or cell lysate or extract.
Western Blot Dilution: 1:1,000
ELISA Dilution: 1:15,000-1:25,000
Other: User Optimized

限制

仅限研究用
状态

Liquid

浓度

1.0 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
Wang, Matsuura, He, Liu: "Transforming growth factor-{beta}-inducible phosphorylation of Smad3." in: The Journal of biological chemistry, Vol. 284, Issue 15, pp. 9663-73, (2009) (PubMed).
抗原

SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

别名

SMAD3 (SMAD3 产品)

背景

Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Smad3 (also known as Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. These activators exert diverse effects on a wide array of cellular processes. The Smad proteins mediate much of the signaling responses induced by the TGF-beta superfamily. Activated type I receptor phosphorylates receptor-activated Smads (R-Smads) at their c-terminal two extreme serines in the S-S-X-S motif, e.g. Smad2 and Smad3 proteins in the TGF-b pathway, or Smad1, Smad5 or Smad8 in the bone morphogenic protein or BMP pathway. Upon phosphorylation R-Smads are translocated into nucleus, where they regulate transcription of target genes. Based on microarray and animal model experiments, Smad3 accounts for at least 80 % of all TGF-b-mediated response.

基因ID

4088

NCBI登录号

NP_005893

UniProt

P84022

途径

Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy