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SMAD3 抗体 (Internal Region)

This anti-SMAD3 antibody is a 兔 多克隆 antibody detecting SMAD3 in WB 和 ELISA. Suitable for 人. This Primary Antibody has been cited in 1 publication.
Rockland
产品编号 ABIN1043911
Supplier Product No.: 600-401-e70
发货至: 中国

Quick Overview for SMAD3 抗体 (Internal Region) (ABIN1043911)

抗原

See all SMAD3 抗体
SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

适用

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宿主

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克隆类型

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多克隆

标记

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This SMAD3 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA
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    Internal Region

    Supplier Product No.

    600-401-e70

    Supplier

    Rockland

    原理

    SMAD3 Antibody

    交叉反应 (详细)

    SMAD3 Antibody is directed against human Smad3 protein.

    产品特性

    Synonyms: rabbit anti-SMAD3 antibody, SMAD-3, SMAD 3, mothers against decapentaplegic homolog 3 antibody, MAD homolog 3, Mothers against DPP homolog 3, SMAD family member 3, MADH3, MADH 3, JV15-2

    纯化方法

    The product was affinity purified from monospecific antiserum by immunoaffinity purification.

    过滤

    Sterile filtered

    免疫原

    Immunogen: SMAD3 Antibody was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of human Smad3 protein surrounding amino acid residue 179.

    Immunogen Type: Conjugated Peptide

    亚型

    IgG
  • 应用备注

    Application Note: SMAD3 Antibody has been tested for use in ELISA and by western blot. This antibody is suitable in immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48.1 kDa in size corresponding to human Smad3 protein by western blotting in the appropriate tissue or cell lysate or extract.

    Western Blot Dilution: 1:1,000

    ELISA Dilution: 1:15,000-1:25,000

    Other: User Optimized

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1.0 mg/mL

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    有效期

    12 months
  • Wang, Matsuura, He, Liu: "Transforming growth factor-{beta}-inducible phosphorylation of Smad3." in: The Journal of biological chemistry, Vol. 284, Issue 15, pp. 9663-73, (2009) (PubMed).

  • 抗原

    SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

    别名

    SMAD3

    背景

    Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Smad3 (also known as Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. These activators exert diverse effects on a wide array of cellular processes. The Smad proteins mediate much of the signaling responses induced by the TGF-beta superfamily. Activated type I receptor phosphorylates receptor-activated Smads (R-Smads) at their c-terminal two extreme serines in the S-S-X-S motif, e.g. Smad2 and Smad3 proteins in the TGF-b pathway, or Smad1, Smad5 or Smad8 in the bone morphogenic protein or BMP pathway. Upon phosphorylation R-Smads are translocated into nucleus, where they regulate transcription of target genes. Based on microarray and animal model experiments, Smad3 accounts for at least 80 % of all TGF-b-mediated response.

    基因ID

    4088

    NCBI登录号

    NP_005893

    UniProt

    P84022

    途径

    Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy
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