Interleukin 17a 抗体 (HRP)
Quick Overview for Interleukin 17a 抗体 (HRP) (ABIN1043841)
抗原
See all Interleukin 17a (IL17A) 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 212-403-b32
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Supplier
- Rockland
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原理
- IL-17A Antibody Peroxidase Conjugated
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交叉反应 (详细)
- In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant rat IL17-A in cell supernatants and certain body fluids.
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产品特性
- Synonyms: rabbit anti-IL-17A Peroxidase Conjugated Antibody, rabbit anti-Interleukin-17A Peroxidase Conjugated Antibody, Interleukin17, IL-17, Cytotoxic T-lymphocyte-associated antigen 8 and CTLA-8
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纯化方法
- This purified antibody has been heated to 56°C for 30 minutes.
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免疫原
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Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant rat IL17-A protein.
Immunogen Type: Recombinant Protein
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亚型
- IgG
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应用备注
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Immunohistochemistry Dilution: 1:500-1:2,500
Application Note: This purified antibody has been tested for use in western blotting and suitable in ELISA. By western blot a band approximately 30.0 kDa in size corresponding to rat IL17-A protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: 1:1,000-1:5,000
ELISA Dilution: 1:10,000 - 1:50,000
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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CTLA-8, cloned from an activated T cell, bearing AU-rich messenger RNA instability sequences, and homologous to a herpesvirus saimiri gene." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 12, pp. 5445-56, (1993) (PubMed).
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CTLA-8, cloned from an activated T cell, bearing AU-rich messenger RNA instability sequences, and homologous to a herpesvirus saimiri gene." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 12, pp. 5445-56, (1993) (PubMed).
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- Interleukin 17a (IL17A) (Interleukin 17A (IL17A))
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别名
- Il17a
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背景
- Background: Rat IL17-A (also known as Interleukin-17, Cytotoxic T-lymphocyte-associated antigen 8 and CTLA-8) is a proinflammatory cytokine member of a six-species family of proteins (IL-17A-17F). Rat IL-17A protein is a homodimer consisting of two 134 amino acids peptides. IL-17A is secreted mainly by activated CD4+ and CD8+ T lymphocytes and acts through its receptor, IL-17R, to induce the expression of many mediators of inflammation, most strikingly, those that are involved in the proliferation, maturation and chemotaxis of neutrophils. Elevated levels of IL-17A have been associated with several conditions, including rheumatoid arthritis, airway inflammation, allograft rejection, inflammatory bowel disease, psoriasis, cancer and multiple sclerosis. There is 58 % identity between the amino acid sequence of human and rat IL-17A.
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基因ID
- 301289
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UniProt
- Q61453
抗原
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