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Interleukin 17a 抗体 (HRP)

Cited in 1 publication. This anti-Interleukin 17a antibody is a 兔 多克隆 antibody detecting Interleukin 17a in ELISA, WB 和 IHC. Suitable for 小鼠 和 大鼠.
Rockland
产品编号 ABIN1043841
Supplier Product No.: 212-403-b32
发货至: 中国
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Quick Overview for Interleukin 17a 抗体 (HRP) (ABIN1043841)

抗原

See all Interleukin 17a (IL17A) 抗体
Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

适用

  • 104
  • 55
  • 18
  • 7
  • 6
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
小鼠, 大鼠

宿主

  • 59
  • 54
  • 20
  • 15
  • 5
  • 2

克隆类型

  • 90
  • 64
  • 1
多克隆

标记

  • 80
  • 19
  • 18
  • 13
  • 10
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This Interleukin 17a antibody is conjugated to HRP

应用范围

  • 71
  • 51
  • 42
  • 17
  • 15
  • 11
  • 10
  • 8
  • 8
  • 7
  • 5
  • 5
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
  • Supplier Product No.

    212-403-b32

    Supplier

    Rockland

    原理

    IL-17A Antibody Peroxidase Conjugated

    交叉反应 (详细)

    In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant rat IL17-A in cell supernatants and certain body fluids.

    产品特性

    Synonyms: rabbit anti-IL-17A Peroxidase Conjugated Antibody, rabbit anti-Interleukin-17A Peroxidase Conjugated Antibody, Interleukin17, IL-17, Cytotoxic T-lymphocyte-associated antigen 8 and CTLA-8

    纯化方法

    This purified antibody has been heated to 56°C for 30 minutes.

    免疫原

    Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant rat IL17-A protein.

    Immunogen Type: Recombinant Protein

    亚型

    IgG
  • 应用备注

    Immunohistochemistry Dilution: 1:500-1:2,500

    Application Note: This purified antibody has been tested for use in western blotting and suitable in ELISA. By western blot a band approximately 30.0 kDa in size corresponding to rat IL17-A protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

    Western Blot Dilution: 1:1,000-1:5,000

    ELISA Dilution: 1:10,000 - 1:50,000

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

    储存液

    Gentamicin sulfate

    注意事项

    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    有效期

    12 months
  • Rouvier, Luciani, Mattéi, Denizot, Golstein: "CTLA-8, cloned from an activated T cell, bearing AU-rich messenger RNA instability sequences, and homologous to a herpesvirus saimiri gene." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 12, pp. 5445-56, (1993) (PubMed).

  • 抗原

    Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

    别名

    Il17a

    背景

    Background: Rat IL17-A (also known as Interleukin-17, Cytotoxic T-lymphocyte-associated antigen 8 and CTLA-8) is a proinflammatory cytokine member of a six-species family of proteins (IL-17A-17F). Rat IL-17A protein is a homodimer consisting of two 134 amino acids peptides. IL-17A is secreted mainly by activated CD4+ and CD8+ T lymphocytes and acts through its receptor, IL-17R, to induce the expression of many mediators of inflammation, most strikingly, those that are involved in the proliferation, maturation and chemotaxis of neutrophils. Elevated levels of IL-17A have been associated with several conditions, including rheumatoid arthritis, airway inflammation, allograft rejection, inflammatory bowel disease, psoriasis, cancer and multiple sclerosis. There is 58 % identity between the amino acid sequence of human and rat IL-17A.

    基因ID

    301289

    UniProt

    Q61453
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