GDF15 抗体 (Biotin)
(1 reference)
Quick Overview for GDF15 抗体 (Biotin) (ABIN1043807)
抗原
See all GDF15 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 209-406-b88
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Supplier
- Rockland
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原理
- GDF15 Antibody Biotin Conjugated
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交叉反应 （详细）
- This antibody reacts with endogenous NAG-1 protein from human tissues. A BLAST analysis using the immunizing recombinant protein suggests reactivity with NAG-1 from human and chimpanzee based on 100 % and 98 % homology, respectively, and with macaque based on an 94 % homology, and with mouse and rat based on 70 % homology with the immunizing sequence.
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产品特性
- Synonyms: rabbit anti-NAG-1 biotin Conjugated Antibody, NAG1, GDF15, MIC-1, nonsteroidal anti-inflammatory drug-activated gene, NSAID-activated gene 1 protein, growth differentiation factor 15, macrophage inhibitory compound 1, prostate-derived factor
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免疫原
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Immunogen: This Protein-A purified antibody was prepared by repeated immunizations with an MBP-tagged recombinant protein produced in E.coli corresponding to mature human NAG-1 protein. Cross reactivity to MBP was removed via cross-adsorption chromatography.
Immunogen Type: Recombinant Protein
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亚型
- IgG
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anti-Growth Differentiation Factor 15 (GDF15) (C-Term) antibody
Verified GDF15 适用: 人 WB, ELISA, IHC, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Growth Differentiation Factor 15 (GDF15) (AA 51-79) antibodyGDF15 适用: 人 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Growth Differentiation Factor 15 (GDF15) (AA 221-308) antibodyGDF15 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Growth Differentiation Factor 15 (GDF15) (AA 30-308) antibodyGDF15 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Growth Differentiation Factor 15 (GDF15) (C-Term) antibody (Biotin)GDF15 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal Biotin
anti-Growth Differentiation Factor 15 (GDF15) (AA 195-308) antibodyGDF15 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Growth Differentiation Factor 15 (GDF15) (AA 16-143) antibodyGDF15 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Growth Differentiation Factor 15 (GDF15) (AA 198-308) antibodyGDF15 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Growth Differentiation Factor 15 (GDF15) (N-Term) antibodyGDF15 适用: 人 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Growth Differentiation Factor 15 (GDF15) (AA 51-79), (N-Term) antibodyGDF15 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB30209 unconjugated
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应用备注
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Immunohistochemistry Dilution: 1:1,000-1:5,000
Application Note: This Protein-A purified, MBP-cross-adsorbed NAG1/GDF15 Biotin conjugated antibody has ben tested by Western blot of human and mouse NAG-1 protein and is suitable for ELISA. For detection of NAG-1 in human serum, a sandwich ELISA is suggested using this antibody in combination with anti-NAG1/GDF15 C-terminal specific antibodies. This antibody is useful in dual antibody immunometric assays (EIA). Specific conditions for reactivity should be optimized by the end user. Expect bands in western blots of approximately 14 kDa in size corresponding to NAG-1 monomer using the appropriate cell lysate or extract.
Western Blot Dilution: 1:2,000-1:10,000
ELISA Dilution: 1:20,000 - 1:100,000
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Changes in gene expression contribute to cancer prevention by COX inhibitors." in: Progress in lipid research, Vol. 45, Issue 1, pp. 1-16, (2006) (PubMed).
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: "Changes in gene expression contribute to cancer prevention by COX inhibitors." in: Progress in lipid research, Vol. 45, Issue 1, pp. 1-16, (2006) (PubMed).
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- GDF15 (Growth Differentiation Factor 15 (GDF15))
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别名
- GDF15
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背景
- Background: Non-steroidal anti-inflammatory drug (NSAID) activated gene (NAG-1) is a member of the transforming growth factor-beta (TGF-beta) superfamily. NAG-1 is also known as Macrophage Inhibitory Cytokine-1 (MIC-1), Growth Differentiation Factor 15 (GDF15), Placental Bone Morphogenetic Protein (PLAB), or Prostate Derived Factor (PDF). NAG-1 is expressed in human placenta, prostate and colon. It possesses antitumorigenic and proapoptotic activities. NAG-1 expression is dramatically increased in inflammation, injury and malignancy. Increase of NAG-1 expression is a feature of many cancers including breast, colon, pancreas and prostate. In a number of studies, NAG-1 expression was increased by a number of NSAIDs. This increase in expression may correlate with the chemopreventive effect NSAIDs seem to have with certain cancers. NAG-1 expression is also induced by PPAR gamma ligands and by several dietary compounds such as conjugated linoleic acids (CLAs), naturally occurring fatty acids in ruminant food products, indoles, epicatechin gallate, and genistein. Induced expression of NAG-1 results in stimulation of apoptosis and inhibition of cell growth. Inhibition of NAG-1 induced expression by small interference RNA (siRNA) results in repression of induced apoptosis. NAG-1 expression is regulated by a numbers of transcription factors such as ERG-1 and Sp1. EGR-1 may be necessary for NSAID-induced NAG-1 expression. The study of expression of NAG-1 proteins, including variants, is important to define their potential role as serum biomarkers for cancer diagnosis, treatment monitoring, epidemiology study, and nutrition surveys.
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基因ID
- 9518
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UniProt
- Q99988
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途径
- SARS-CoV-2 Protein Interactome
抗原
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