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Interferon gamma 抗体

Rockland 201-401-c41 IFNG 适用: Cow WB, ELISA 宿主: 兔 Polyclonal unconjugated
Rockland
产品编号 ABIN1043777
Supplier Product No.: 201-401-c41
发货至: 中国
  • 抗原 See all Interferon gamma (IFNG) 抗体
    Interferon gamma (IFNG)
    适用
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    This Interferon gamma antibody is un-conjugated
    应用范围
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    Western Blotting (WB), ELISA
    Supplier Product No.
    201-401-c41
    Supplier
    Rockland
    原理
    Interferon Gamma Antibody
    交叉反应 (详细)
    This antibody is specific for bovine IFN gamma protein.
    产品特性
    Synonyms: rabbit anti-IFN gamma, rabbit anti-Interferon gamma, IFNG, BoIFNG
    纯化方法
    This product was protein A purified from monospecific antiserum by chromatography.
    免疫原

    Immunogen: This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast corresponding to the 143 amino acids of the mature Bovine IFN Gamma protein.

    Immunogen Type: Recombinant Protein

    亚型
    IgG
    Top Product
    Discover our top product IFNG Primary Antibody
  • 应用备注

    Application Note: This protein A purified IFN-gamma antibody has been tested by ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 16.9 kDa in size corresponding to bovine IFN gamma by western blotting in the appropriate cell lysate or extract.

    Western Blot Dilution: 1:500-1:2,000

    ELISA Dilution: 1:20,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    1.59 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • George, Ma, Motyka, Shi, Liu, Griebel: "A dendritic cell-targeted chimeric hepatitis B virus immunotherapeutic vaccine induces both cellular and humoral immune responses in vivo." in: Human vaccines & immunotherapeutics, Vol. 16, Issue 4, pp. 779-792, (2020) (PubMed).

    Schroder, Hertzog, Ravasi, Hume: "Interferon-gamma: an overview of signals, mechanisms and functions." in: Journal of leukocyte biology, Vol. 75, Issue 2, pp. 163-89, (2004) (PubMed).

  • 抗原
    Interferon gamma (IFNG)
    别名
    IFN gamma (IFNG 产品)
    背景
    Background: Interferon-gamma (IFN-gamma) is a dimerized soluble cytokine that is the only member of the type II class interferon. This interferon was originally called macrophage-activating factor, a term now used to describe a larger family of proteins to which IFN-gamma belongs. IFN-gamma, or type II interferon, is a cytokine that is critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Aberrant IFN-gamma expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFN-gamma in the immune system stems in part from its ability to inhibit viral replication directly, but, most important, derives from its immunostimulatory and immunomodulatory effects. IFN-gamma is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.
    基因ID
    281237
    NCBI登录号
    NP_776511
    UniProt
    A9QXB7
    途径
    Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy
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