Ubiquitin B 抗体
(1 reference)
Quick Overview for Ubiquitin B 抗体 (ABIN108010)
抗原
See all Ubiquitin B (UBB) 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-401-431
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Supplier
- Rockland
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原理
- Ubiquitin Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
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产品特性
- Synonyms: rabbit anti-Ubiquitin Antibody, FLJ25987 antibody, MGC8385 antibody, Polyubiquitin B antibody, RPS27A antibody, UBA52 antibody, UBA80 antibody, UBB antibody, UBC antibody, UBCEP1 antibody
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纯化方法
- This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: This purified antibody was prepared from rabbit serum after repeated immunizations with ubiquitin coupled to rabbit IgG using glutaraldehyde.
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Ubiquitin B (UBB) (AA 1-119) antibody
UBB 适用: 人 WB, IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal UBB-1748 unconjugated
anti-Ubiquitin B (UBB) (AA 1-119) antibodyUBB 适用: 人 WB, IHC, ELISA, StM, Coat 宿主: 小鼠 Monoclonal UBB-2122 unconjugated
anti-Ubiquitin B (UBB) antibodyUBB 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 3C12 unconjugated
anti-Ubiquitin B (UBB) antibodyUBB 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-Ubiquitin B (UBB) (AA 1-76) antibodyUBB 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1F5 unconjugated
anti-Ubiquitin B (UBB) (AA 77-152) antibodyUBB 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin B (UBB) (AA 103-228) antibodyUBB 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin B (UBB) (AA 1-119) antibodyUBB 适用: 人 IHC, StM 宿主: 兔 Monoclonal UBB-3143R unconjugated Recombinant Antibody
anti-Ubiquitin B (UBB) antibodyUBB 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Ubiquitin B (UBB) (AA 25-57), (N-Term) antibodyUBB 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal RB49507 unconjugated
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应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: This purified polyclonal antibody reacts with ubiquitin by ELISA and western blot. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. For detection of free Ub by western blotting use Tris-Tricine SDS-PAGE and autoclaved nitrocellulose filters after the transfer and before blocking and addition of anti-Ub antibodies. Details on western blotting procedures are found in Mimnaugh et al., (1999 and 2002).
Western Blot Dilution: 1:200 - 1:1000
ELISA Dilution: 1:1,000 - 1:5,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "The measurement of ubiquitin and ubiquitinated proteins." in: Electrophoresis, Vol. 20, Issue 2, pp. 418-28, (1999) (PubMed).
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: "The measurement of ubiquitin and ubiquitinated proteins." in: Electrophoresis, Vol. 20, Issue 2, pp. 418-28, (1999) (PubMed).
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- Ubiquitin B (UBB)
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别名
- UBB
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背景
- Background: Ubiquitin (Ub) is a small, 76-residue, protein (8.5 kDa) found both as free monomer and covalently attached to itself and other proteins in eukaryotic cells. Free Ub is a very compact and stable molecule that is easily refolded after being denatured. It is therefore recommended that for detection of free Ub on Westerns, the Tris-Tricine SDS-PAGE is used and nitrocellulose filters are autoclaved after the transfer and before blocking and addition of anti-Ub antibodies. The C-terminus of ubiquitin forms an isopeptide bond with the e-amino group of a lysine side chain in a target protein. In this way proteins can be covalently modified by the addition of ubiquitin which may alter the target protein's function. Monoubiquitination generally targets proteins for internalization, endocytosis and lysosomal degradation, or modifies the surface charge of histones and affects chromatin compaction. Conjugation of ubiquitin (Ub) involves a three-step mechanism whereby specific enzymes (or enzyme complexes) activate and covalently link Ub to their substrates.
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基因ID
- 7314
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NCBI登录号
- NP_001268645
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UniProt
- P0CG47
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途径
- Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, Toll-Like Receptors Cascades, Synthesis of DNA, Autophagy, EGFR Downregulation, Ubiquitin Proteasome Pathway
抗原
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