TIMP4 抗体
(1 reference)
Quick Overview for TIMP4 抗体 (ABIN1043743)
抗原
See all TIMP4 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-301-e65
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Supplier
- Rockland
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原理
- TIMP4 Antibody
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交叉反应 （详细）
- TIMP4 antibody is specific for human TIMP4 protein.
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产品特性
- Synonyms: mouse anti-TIMP4 antibody, Tissue inhibitor of metalloproteinase-4, Metalloproteinase inhibitor 4, TIMP-4, TIMP 4, TIMP4 antibody, anti-TIMP-4 antibody
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纯化方法
- Anti-TIMP4 was purified from clarified mouse ascetic fluid by Protein A chromatography followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-TIMP4 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with full length human TIMP4 protein followed by hybridoma development.
Immunogen Type: Recombinant Protein
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亚型
- IgG2b kappa
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anti-TIMP Metallopeptidase Inhibitor 4 (TIMP4) (AA 32-224) antibody
TIMP4 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-TIMP Metallopeptidase Inhibitor 4 (TIMP4) (C-Term) antibodyTIMP4 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-TIMP Metallopeptidase Inhibitor 4 (TIMP4) (AA 31-224) antibodyTIMP4 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-TIMP Metallopeptidase Inhibitor 4 (TIMP4) (C-Term) antibodyTIMP4 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-TIMP Metallopeptidase Inhibitor 4 (TIMP4) (AA 174-224) antibodyTIMP4 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-TIMP Metallopeptidase Inhibitor 4 (TIMP4) (AA 42-224) antibodyTIMP4 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-TIMP Metallopeptidase Inhibitor 4 (TIMP4) (AA 161-224) antibodyTIMP4 适用: 人, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-TIMP Metallopeptidase Inhibitor 4 (TIMP4) (AA 30-224) antibodyTIMP4 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-TIMP Metallopeptidase Inhibitor 4 (TIMP4) (AA 48-74) antibodyTIMP4 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB22433 unconjugated
anti-TIMP Metallopeptidase Inhibitor 4 (TIMP4) (AA 55-82), (Middle Region) antibodyTIMP4 适用: 人, 小鼠 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: 5-10 μg/mL
Application Note: Anti-TIMP4 antibody has been tested for use in ELISA, Native - Western, and IHC (P). SDS-PAGE - Western Blot not useful for endogenous detection. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: 1:2,000 to 1:10,000
ELISA Dilution: 1:20,000 to 1:100,000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Development of a monoclonal antibody that specifically detects tissue inhibitor of metalloproteinase-4 (TIMP-4) in formalin-fixed, paraffin-embedded human tissues." in: Journal of cellular biochemistry, Vol. 110, Issue 5, pp. 1255-61, (2010) (PubMed).
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: "Development of a monoclonal antibody that specifically detects tissue inhibitor of metalloproteinase-4 (TIMP-4) in formalin-fixed, paraffin-embedded human tissues." in: Journal of cellular biochemistry, Vol. 110, Issue 5, pp. 1255-61, (2010) (PubMed).
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- TIMP4 (TIMP Metallopeptidase Inhibitor 4 (TIMP4))
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别名
- TIMP4
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背景
- Background: Tissue inhibitor of metalloproteinase-4 (TIMP-4) is member of the TIMP family of inhibitors of matrix metalloproteinase (MMP) activities. In addition, TIMPs have non-MMP associated functions, including roles in growth promotion, apoptosis, and angiogenesis. All four members of the TIMP gene family form a similar secondary structure due to the formation of six highly conserved disulfide bonds. It has been reported that some TIMPs, TIMP4 included, can bind a tetraspanin in the plasma membrane, initiating an intra-cellular signaling cascade. Elevated levels of TIMP4 have been associated with breast cancer progression and heart disease.
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基因ID
- 7079
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NCBI登录号
- NM_003256
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UniProt
- Q99727
抗原
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