LGR4 抗体 (Internal Region)
(1 reference)
Quick Overview for LGR4 抗体 (Internal Region) (ABIN1043731)
抗原
See all LGR4 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- Internal Region
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Supplier Product No.
- 200-301-b46
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Supplier
- Rockland
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原理
- LGR4 Antibody
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交叉反应 （详细）
- This antibody is specific for human LGR4 protein.
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产品特性
- Synonyms: mouse anti-LGR4 antibody, mouse anti-LGR 4 antibody, leucine-rich repeat-containing G protein-coupled receptor 4
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纯化方法
- Anti-LGR4 Antibody product was purified from concentrated tissue culture supernate by Protein A chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This LGR4 antibody was produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human LGR4 protein. The hybridoma was produced by fusing BALB/c mouse splenocytes and mouse myeloma SP2/O cells using conventional technology.
Immunogen Type: Conjugated Peptide
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亚型
- IgG2b kappa
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anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4) antibody
LGR4 适用: 人 FACS, cELISA 宿主: 大鼠 Monoclonal BBX-1H1 unconjugated
anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4) (AA 234-365) antibodyLGR4 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4) (C-Term) antibodyLGR4 适用: 人, 猴, 兔, Pig, Cow, 犬, 马 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4) (C-Term) antibodyLGR4 适用: 人, 猴, 兔, Hamster IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4) (Cytoplasmic Domain) antibodyLGR4 适用: 人 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4) (AA 852-950) antibodyLGR4 适用: 人 ELISA, WB 宿主: 小鼠 Monoclonal 8F6 unconjugated
anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4) (AA 320-540) antibodyLGR4 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4) (N-Term) antibodyLGR4 适用: 人, 小鼠, 大鼠, 猴, 兔, Pig IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4) (C-Term) antibodyLGR4 适用: 人, 大鼠 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4) antibodyLGR4 适用: 人, 小鼠 FACS, cELISA 宿主: 大鼠 Monoclonal BBX-3A8 unconjugated
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应用备注
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Immunohistochemistry Dilution: 5 μg/mL
Application Note: Anti-LGR4 antibody has been tested by ELISA and western blotting, and is suitable in immunohistochemistry. Expect a band approximately 102 kDa in size corresponding to LGR4 protein by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. Use formalin-fixed paraffin-embedded sections for immunohistochemistry. No pre-treatment of sample is required.
Western Blot Dilution: 1:500 - 1:3,000
ELISA Dilution: 1:20,000 - 1:100,000
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store Anti-LGR4 Monoclonal Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge antibody if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Deletion of G protein-coupled receptor 48 leads to ocular anterior segment dysgenesis (ASD) through down-regulation of Pitx2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 16, pp. 6081-6, (2008) (PubMed).
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: "Deletion of G protein-coupled receptor 48 leads to ocular anterior segment dysgenesis (ASD) through down-regulation of Pitx2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 16, pp. 6081-6, (2008) (PubMed).
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- LGR4 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4))
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别名
- LGR4
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背景
- Background: LGR4, also known as leucine-rich repeat-containing G protein-coupled receptor 4, is a G protein-coupled receptors (GPCRs). GPCRs are membrane bound proteins that play key roles in a variety of physiologic functions. Members of the leucine-rich GPCR (LGR) family, such as GPR48, have multiple N-terminal leucine-rich repeats (LRRs) and a 7-transmembrane domain. LGR4 is an orphan GPCR reported to be expressed in steroidogenic tissues such as placenta, ovary, testis, adrenal, pancreas, prostate, and thyroid, as well as in spinal cord, stomach, heart, and kidney.
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基因ID
- 55366, 157694513
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UniProt
- Q8N537
抗原
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