VCAM1 抗体
(5 references)Quick Overview for VCAM1 抗体 (ABIN1042491)
抗原
See all VCAM1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
特异性
- The antibody binds exclusively to the cytokine-induced endothelial cell adhesion protein, vascular cell adhesion molecule-1 (VCAM-1).
-
产品特性
- Tumour marker
-
纯化方法
- Purified
-
亚型
- IgG1
-
-
anti-Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 1-739) antibody
VCAM1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 1-739) antibodyVCAM1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Vascular Cell Adhesion Molecule 1 (VCAM1) antibodyVCAM1 适用: 人, 犬, 猴 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 3H10 unconjugated
anti-Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 25-183) antibodyVCAM1 适用: 人, 大鼠, 猴 WB, FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 6A11D8 unconjugated
anti-Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 109-318) antibodyVCAM1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 300-400) antibodyVCAM1 适用: 人 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Vascular Cell Adhesion Molecule 1 (VCAM1) antibodyVCAM1 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 6G9 unconjugated
anti-Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 511-682) antibodyVCAM1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 369-658) antibodyVCAM1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 409-682) antibodyVCAM1 适用: 兔 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal 3-2# unconjugated
-
-
应用备注
- 4B9 is suitable for immunoblotting, immunoprecipitation, immunocytochemistry and immuno-histochemistry on frozen tissues and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:25 – 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:1000 for immunoblotting applications.
-
说明
-
4B9 is a mouse IgG1 monoclonal antibody generated by immunization of Balb/c mice with human umbilical vein endothelium (HUVE) that were non-enzymatically harvested after stimulation for 24h with recombinant human (rh) TNF-alpha (10 ng/ml). Splenocytes were fused with the NS-1 nonsecretory murine myeloma line.
-
限制
- 仅限研究用
-
-
-
缓冲液
- PBS containing 0.09% sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C
-
-
-
: "Domains 1 and 4 of vascular cell adhesion molecule-1 (CD106) both support very late activation antigen-4 (CD49d/CD29)-dependent monocyte transendothelial migration." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 6, pp. 3135-4, (1995) (PubMed).
: "Human NK cells expressing alpha 4 beta 1/beta 7 adhere to VCAM-1 without preactivation." in: Scandinavian journal of immunology, Vol. 39, Issue 2, pp. 131-6, (1994) (PubMed).
: "Expression of vascular cell adhesion molecule (VCAM-1) in liver and pancreas allograft rejection." in: The American journal of pathology, Vol. 142, Issue 2, pp. 579-91, (1993) (PubMed).
: "Human vascular endothelial cell adhesion receptors for Plasmodium falciparum-infected erythrocytes: roles for endothelial leukocyte adhesion molecule 1 and vascular cell adhesion molecule 1." in: The Journal of experimental medicine, Vol. 176, Issue 4, pp. 1183-9, (1992) (PubMed).
: "Vascular cell adhesion molecule-1 mediates lymphocyte adherence to cytokine-activated cultured human endothelial cells." in: Blood, Vol. 76, Issue 5, pp. 965-70, (1990) (PubMed).
-
: "Domains 1 and 4 of vascular cell adhesion molecule-1 (CD106) both support very late activation antigen-4 (CD49d/CD29)-dependent monocyte transendothelial migration." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 6, pp. 3135-4, (1995) (PubMed).
-
- VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))
-
别名
- VCAM-1
-
背景
- Vascular cell adhesion molecule-1 (VCAM-1) is induced on human umbilical vein endothelium (HUVE) by recombinant human tumor necrosis factor - alpha (rh TNF-alpha), rh interleukin-1 (IL-1) and lipopolysaccharide (LPS) and is involved in adherence of lymphocytic cell lines to HUVE. VCAM-1 is only minimally expressed on unstimulated endothelium. Molecular cloning and sequencing of VCAM-1 predicts a 69-kDa core protein with 6 potential N-linked glycosylation sites, if the protein is fully glycosylated a mature protein of about 90kDa is yielded. VCAM-1 mediates a component of the adherence of Peripheral Blood Lymphocytes (PBL) to rh TNF-stimulated HUVE, this CD18 independent mechanism of lymphocyte adherence to cytokine stimulated endothelium may be an important pathway of lymphocyte emigration at sites of inflammation and immune reaction.
-
途径
- Carbohydrate Homeostasis
抗原
-
