CD4 抗体
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北京 101111
Quick Overview for CD4 抗体 (ABIN1042376)
抗原
See all CD4 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Mouse Anti Human CD4
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特异性
- CD4 (Edu-2) recognizes the CD4 antigen (a 55 kD glycoprotein).
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产品特性
- CD4 (Cluster of Differentiation 4) is a glycoprotein expressed on the surface of T helper cells, monocytes, macrophages, and dendritic cells. It was discovered in the late 1970s and was originally known as leu-3 and T4 (after the OKT4 monoclonal antibody that reacted with it) before being named CD4 in 1984. In humans, the CD4 protein is encoded by the CD4 gene. CD4 plays a role in the recognition of foreign antigens presented to T cells by MHC class II molecules. Furthermore, this antigen acts as a receptor for HIV-1 by binding the viral protein gp120. The CD4 antigen is present on most thymocytes and a subpopulation of peripheral blood T cells, called T helper cells (Th). In addition, CD4 is expressed on monocytes and weak on macrophages.
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免疫原
- Edu-2 is a mouse monoclonal IgG2a antibody raised against CD4.
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亚型
- IgG2a
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anti-CD4 (CD4) (AA 385-457) antibody
CD4 适用: 人 FACS, WB, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD4 (CD4) antibodyCD4 适用: 人, 小鼠, 大鼠, 猴, 小鸡, 犬, 兔, Cow, 绵羊, 豚鼠, 山羊, Pig, 马, Avian, 驴, Hamster WB, IHC (fro), IHC (p) 宿主: 山羊 Polyclonal unconjugated
anti-CD4 (CD4) (AA 231-330) antibodyCD4 适用: 小鼠 FACS, WB, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD4 (CD4) (N-Term) antibodyCD4 适用: 人 FACS, WB, MS 宿主: 小鼠 Monoclonal MEM-241 unconjugated
anti-CD4 (CD4) antibodyCD4 适用: 人, 小鼠, 大鼠 IHC 宿主: 小鼠 Monoclonal unconjugated
anti-CD4 (CD4) (AA 301-400) antibodyCD4 适用: 人 FACS, WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD4 (CD4) (AA 26-200) antibodyCD4 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-CD4 (CD4) antibody (PE)CD4 适用: 大鼠 FACS 宿主: 小鼠 Monoclonal OX-35 PE
anti-CD4 (CD4) antibodyCD4 适用: 小鼠 FACS, IP, IHC (fro), ICC, Func 宿主: 大鼠 Monoclonal GK1-5 unconjugated
anti-CD4 (CD4) (N-Term) antibody (FITC)CD4 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-241 FITC
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应用备注
- Flow cytometry and immunohistochemistry using frozen and paraffin embedded tissue sections. Optimal antibody dilution should be determined by titration. CD4 (Edu-2) is used in routine blood testing for CD4+ cells and CD4/CD8 ratios (e.g. HIV/AIDS patients) or as part of panels for the detection and differentiation of certain T cell leukemias. All these reagents are effectively formulated for indirect immunofluorescent staining of human blood for flow cytometric analysis using 10 μL/106 leukocytes. Staining with clone Edu-2 (CD4) monoclonal antibodies by flow cytometry analysis of normal blood cells is illustrated. Indirect staining was performed using 10 μL of the purified monoclonal antibody with RaM FITC conjugate and 100 μL blood sample. However each investigator should titer the antibody in their application to determine the optimal per-test amount. CD4 is also used in studies of functional activity of Th-cells in bacterial and viral infections, development of auto-immune diseases, transplant rejection, immune protection in response to allergens or allergenic reactivity.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Each vial contains 1 mL of 100 μg/mL purified monoclonal antibody in 0.01 M sodium phosphate, 0.15 M NaCl, 0.2 % BSA, 0.09 % sodium azide, pH 7.3.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
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: "Structure, function, and serology of the T-cell antigen receptor complex." in: Annual review of immunology, Vol. 5, pp. 503-40, (1987) (PubMed).
: "Human T lymphocyte proliferation induced by a pan-T monoclonal antibody (anti-Leu 4): heterogeneity of response is a function of monocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 131, Issue 1, pp. 158-64, (1983) (PubMed).
: "T-lymphocyte reconstitution in recipients of bone marrow transplants with and without GVHD: imbalances of T-cell subpopulations having unique regulatory and cognitive functions." in: Blood, Vol. 59, Issue 4, pp. 696-701, (1982) (PubMed).
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- CD4
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别名
- CD4
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UniProt
- P01730
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途径
- TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
抗原
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