MAPKAP Kinase 2 抗体 (Internal Region)
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- 抗原 See all MAPKAP Kinase 2 (MAPKAPK2) 抗体
- MAPKAP Kinase 2 (MAPKAPK2) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2))
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抗原表位
- Internal Region
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This MAPKAP Kinase 2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)
- Supplier Product No.
- 600-401-281
- Supplier
- Rockland
- 原理
- MAPKAP Kinase 2 Antibody
- 交叉反应 （详细）
- This affinity-purified antibody is directed against rabbit MAPKAP Kinase 2 and is useful in determining its presence in various assays. This polyclonal anti-MAPKAP Kinase 2 antibody recognizes native and over-expressed protein found in various tissues and extracts.
- 产品特性
- Synonyms: rabbit anti-MAPKAP kinase 2 antibody, MAP kinase-activated protein kinase 2, MAPK-activated protein kinase 2, MAPKAP kinase-2, MK2, mitogen-activated protein kinase-activated protein kinase-2, MAPKAP-K2, MAPKAPK-2, MK-2, MK2
- 纯化方法
- Affinity purified antibody
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: This antibody was affinity purified from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of rabbit MAPKAP Kinase 2 conjugated to KLH using maleimide. A terminal cysteine residue was added to facilitate coupling.
Immunogen Type: Conjugated Peptide
- 亚型
- IgG
- Top Product
- Discover our top product MAPKAPK2 Primary Antibody
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anti-Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2) (AA 139-367) antibody
MAPKAPK2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2) (AA 302-400) antibodyMAPKAPK2 适用: 人 WB, ELISA, IHC (p), IP, IF, PLA 宿主: 小鼠 Monoclonal 2B3 unconjugated
anti-Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2) (AA 266-352) antibodyMAPKAPK2 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3B8 unconjugated
anti-Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2) (Internal Region) antibodyVerified MAPKAPK2 适用: 人, 小鼠 WB, IHC, ELISA, IP 宿主: 山羊 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2) (AA 301-400) antibodyMAPKAPK2 适用: 大鼠 WB, ELISA, IF (p), IHC (p), IF (cc), FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2) (pThr222) antibodyMAPKAPK2 适用: 小鼠, 大鼠, 猴 WB, ELISA, IF (p), IHC (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2) (pThr334) antibodyMAPKAPK2 适用: 人, 小鼠, 大鼠 IF (p), IHC (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2) antibodyMAPKAPK2 适用: 人 WB, IHC, ELISA 宿主: 兔 Monoclonal 4H8 unconjugated Recombinant Antibody
anti-Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2) (pThr334) antibodyMAPKAPK2 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2) (pThr334) antibodyMAPKAPK2 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: Anti-MAPKAP kinase 2 antibody is tested by western blotting and suitable for ELISA, immunoprecipitation and kinase assays. Although not tested, this antibody is likely functional in immunohistochemistry and other immunological methods. The antibody detects a single 44 kDa protein in crude extracts prepared either from unstimulated (p/n w09-000-364) or TNFα stimulated ((p/n w09-000-367) human HeLa cell lysates. A 1:15,000 dilution is recommended for ELISA. The researcher should determine optimal titers for other applications.
Western Blot Dilution: 1:2,000
Immunoprecipitation Dilution: User Optimized
ELISA Dilution: 1:15,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.9 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- MAPKAP Kinase 2 (MAPKAPK2) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2))
- 别名
- MAPKAPK2 (MAPKAPK2 产品)
- 背景
- Background: MAPKAP kinase-2 (or MK2) is an acronym for mitogen-activated protein kinase-activated protein kinase-2. This enzyme is phosphorylated and activated by Erks and p38 MAPK in vitro. While multiple residues of MK2 are phosphorylated in vivo in response to stress, only four of the residues Thr25, Thr222, Ser272 and Thr334 are phosphorylated by p38 in an in vitro kinase assay. MK2 is serine-threonine protein kinase that contains a proline rich sequence and two putative SH3-binding sites. MK2 phosphorylates downstream components on a consensus Pro-X-Ser/Thr-Pro motif. The best-characterized MK2 substrate is hsp27. Although unknown, the physiological function of MK2 may be to increase stress resistance. MK2 is an essential component in the inflammatory response that regulates biosynthesis of TNFα at a post-transcriptional level and MK2 is rapidly phosphorylated and activated in response to cytokines, stress and chemotactic factors.
- 基因ID
- 9261
- UniProt
- P49137
- 途径
- MAPK Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades
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