LIM Domain Binding 1 Protein 抗体 (C-Term)
Quick Overview for LIM Domain Binding 1 Protein 抗体 (C-Term) (ABIN103458)
抗原
See all LIM Domain Binding 1 Protein (LDB1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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Supplier Product No.
- 600-401-350
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Supplier
- Rockland
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原理
- LDB1 Antibody
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交叉反应 （详细）
- This affinity purified antibody is directed against the mouse LDB1protein.
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产品特性
- Synonyms: rabbit anti-LDB1 antibody, rabbit anti-LDB1/CLIM2 antibody, rabbit anti-CLIM2 antibody, LDB-1, CLIM-2, LIM domain-binding protein 1, Carboxyl-terminal LIM domain-binding protein 2, LIM domain-binding factor CLIM2, Nuclear LIM interactor
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纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region of mouse LDB1 protein.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-LIM Domain Binding 1 (LDB1) antibody
KO Validated LDB1 适用: 人 WB, IP, IHC (p), ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-LIM Domain Binding 1 (LDB1) (AA 1-50) antibodyLDB1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-LIM Domain Binding 1 (LDB1) (AA 50-375) antibodyLDB1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-LIM Domain Binding 1 (LDB1) antibodyLDB1 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-LIM Domain Binding 1 (LDB1) (AA 303-352) antibodyLDB1 适用: 人, 小鼠, 大鼠, 斑马鱼, 犬, Cow, 豚鼠, 马, 兔, 小鸡, 非洲爪蟾, Pig, Bat, Hamster, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-LIM Domain Binding 1 (LDB1) (AA 1-110) antibodyLDB1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2G9 unconjugated
anti-LIM Domain Binding 1 (LDB1) (AA 112-411) antibodyLDB1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-LIM Domain Binding 1 (LDB1) (AA 1-110) antibodyLDB1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-LIM Domain Binding 1 (LDB1) (AA 251-300) antibodyLDB1 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 马, 兔, 小鸡, 非洲爪蟾, Pig, Bat, Hamster, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-LIM Domain Binding 1 (LDB1) (AA 336-368), (C-Term) antibodyLDB1 适用: 斑马鱼 WB 宿主: 兔 Polyclonal RB47030 unconjugated
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应用备注
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Application Note: This affinity purified antibody has been tested for use in ELISA, western blot and CHIP. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 43 kDa in size corresponding to LDB1 by western blotting in the appropriate cell lysate or extract. This antibody has been used in a ChIP assay using murine erythroleukemia (MEL) cells. The test sequence was the upstream enhancer of the GATA-1 gene, a putative LDB1 binding region as suggested by Orkin et al. Anti-LDB1 was used successfully in ChIP assays to precipitate a roughly 4-fold enrichment at the GATA1-HS1 enhancer element in DMSO-induced murine erythroleukemia cells. We suggest using 20 μg for 10E8 cells for ChIP.
ChIP Dilution: User Optimized
Western Blot Dilution: 1:500 - 1:3,000
ELISA Dilution: 1:425,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.80 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- LIM Domain Binding 1 Protein (LDB1) (LIM Domain Binding 1 (LDB1))
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别名
- Ldb1
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背景
- Background: LDB1 is also known as CLIM 2, LIM Domain Binding 1, NLI and Nuclear LIM Domain Interactor. The LIM-domain binding protein binds to the LIM domain of LIM homeodomain proteins which are transcriptional regulators of development. Nuclear LIM interactor (NLI) / LIM domain-binding protein 1 (LDB1) is located in the nuclei of neuronal cells during development, it is co-expressed with Isl1 in early motor neuron differentiation and has a suggested role in the Isl1 dependent development of motor neurons. It is suggested that these proteins act synergistically to enhance transcriptional efficiency by acting as co-factors for LIM homeodomain and Otx class transcription factors, both of which have essential roles in development.
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基因ID
- 16825, 6754520
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UniProt
- P70662
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途径
- Stem Cell Maintenance, Chromatin Binding
抗原
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