LPO 抗体
Quick Overview for LPO 抗体 (ABIN103436)
抗原
See all LPO 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-601-146-0100
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Supplier
- Rockland
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原理
- Lactoperoxidase Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Lactoperoxidase [Bovine Milk].
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产品特性
- Synonyms: sheep anti-Lactoperoxidase Antibody, LPO antibody, Salivary peroxidase antibody, SAPX antibody, SPO antibody
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纯化方法
- Anti-LACTOPEROXIDASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Lactoperoxidase [Bovine Milk]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Lactoperoxidase (LPO) (AA 351-589) antibody
LPO 适用: 大鼠 WB, IP, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lactoperoxidase (LPO) (AA 305-498) antibodyLPO 适用: 小鼠 WB, IP, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lactoperoxidase (LPO) (AA 244-346) antibodyLPO 适用: 人 WB, IP, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lactoperoxidase (LPO) (AA 351-589) antibodyLPO 适用: 大鼠 WB, IP, IHC, ICC 宿主: 小鼠 Monoclonal 15# unconjugated
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应用备注
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Application Note: Lactoperoxidase antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Lactoperoxidase [Bovine Milk] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Rabbit) code #613-4302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 10.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- LPO (Lactoperoxidase (LPO))
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别名
- Lactoperoxidase
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背景
- Background: Lactoperoxidase is an antimicrobial agent which utilizes hydrogen peroxide and thiocyanate (SCN) to generate the antimicrobial substance hypothiocyanous acid (HOSCN). It may protect the udder from infection and promote growth in newborn calves. It inhibits growth of the following bacterial species: E.coli, K.pneumoniae, P.aeruginosa, S.sonnei, S.saphrophyticus, S.epidermidis, and S.dysenteriae.
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基因ID
- 280844
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UniProt
- P80025
抗原
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