KLH 抗体 (HRP)
This anti-KLH antibody is a 兔 多克隆 antibody detecting KLH in ELISA 和 WB. Suitable for Keyhole Limpet.
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Quick Overview for KLH 抗体 (HRP) (ABIN103381)
抗原
See all KLH 抗体
KLH
(Keyhole Limpet Hemocyanin (KLH))
适用
Keyhole Limpet
宿主
兔
克隆类型
多克隆
标记
This KLH antibody is conjugated to HRP
应用范围
ELISA, Western Blotting (WB)
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Supplier Product No.
- 600-403-466
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Supplier
- Rockland
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产品特性
- Concentration Definition: by UV absorbance at 280 nm
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免疫原
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KLH (Keyhole Limpet Hemocyanin
Immunogentype:Native -
亚型
- IgG
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anti-Keyhole Limpet Hemocyanin (KLH) antibody
KLH 适用: Keyhole Limpet ELISA, WB, IL 宿主: 兔 Polyclonal unconjugated
anti-Keyhole Limpet Hemocyanin (KLH) antibodyKLH 适用: Keyhole Limpet ELISA, WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Anti-KLH Antibody Peroxidase Conjugated antibody has been assayed against 1.0 ug of KLH in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:60,000 is suggested for this product. Anti-KLH Antibody is suitable for western blotting to detect a single band of the expected apparent molecular weight and for ELISA. Peroxidase conjugated anti-KLH antibody is suitable for use with streptavidin reporter systems. Researchers should determine optimal titers for applications that are not stated below.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Restore with deionized water (or equivalent)
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浓度
- 1.0 mg/mL
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
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储存液
- Gentamicin sulfate
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注意事项
- Do NOT add Sodium Azide!
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储存条件
- 4 °C
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- KLH (Keyhole Limpet Hemocyanin (KLH))
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背景
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KLH Antibody, Peroxidase Conjugated, detects Keyhole Limpet Hemocyanin protein which is a very large, heterogeneous glycosylated protein consisting of subunits with a molecular weight of 350,000 and 390,000. KLH readily forms aggregates with molecular weights of 4,500,000-13,000,000 daltons. Each domain of a KLH subunit contains two copper atoms that together bind a single oxygen molecule (O2). When oxygen is bound to hemocyanin, the molecule takes on a distinctive transparent, opalescent blue color. The KLH protein is potently immunogenic yet safe in humans and is therefore highly prized as a vaccine carrier protein. The large and highly glycosylated KLH protein cannot be reproduced synthetically. It is available only as a purified biological product from the Keyhole Limpet Megathura crenulata. KLH is being tested in a variety of cancer vaccines, including non-Hodgkins lymphoma, cutaneous melanoma, breast and bladder cancer.
Synonyms: HRP, Keyhole Limpet Hemocyanin
抗原
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