HMGN1 抗体
Quick Overview for HMGN1 抗体 (ABIN103313)
抗原
See all HMGN1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
Supplier Product No.
- 200-301-278
-
Supplier
- Rockland
-
原理
- IPO-38 Antibody
-
交叉反应 （详细）
- This antigen was detected after 12h of PHA-induced activation in the early G1 phase but absent in non-stimulated lymphocytes.
-
产品特性
- Synonyms: mouse anti-IPO38 antibody, mouse anti-HMGN1 antibody, mouse anti-HMG14 antibody, Non-histone chromosomal protein HMG-14 antibody, High-mobility group nucleosome-binding domain-containing protein 1 antibody
-
纯化方法
- Anti-IPO-38 monoclonal antibody was purified by Protein A chromatography.
-
过滤
- Sterile filtered
-
免疫原
-
Immunogen: A BALB/c mouse was immunized with spleen cells of a human patient with hairy cell leukemia.
Immunogen Type: Native Protein
-
亚型
- IgM kappa
-
-
anti-High Mobility Group Nucleosome Binding Domain 1 (HMGN1) antibody
HMGN1 适用: 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Nucleosome Binding Domain 1 (HMGN1) (AA 1-96) antibodyHMGN1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Nucleosome Binding Domain 1 (HMGN1) (AA 36-85) antibodyHMGN1 适用: 人, 小鼠, 大鼠, Cow, 马, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Nucleosome Binding Domain 1 (HMGN1) (AA 1-100) antibodyHMGN1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Nucleosome Binding Domain 1 (HMGN1) (C-Term) antibodyHMGN1 适用: 人, Cow, Pig, 马, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Nucleosome Binding Domain 1 (HMGN1) (C-Term) antibodyHMGN1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Nucleosome Binding Domain 1 (HMGN1) (Middle Region) antibodyHMGN1 适用: 人, 小鼠, 大鼠, Cow, Pig, 犬, 马, 豚鼠, 兔 WB 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Nucleosome Binding Domain 1 (HMGN1) (Middle Region) antibodyHMGN1 适用: 人, 小鼠, 大鼠, Cow, Pig, 犬, 马, 豚鼠, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Nucleosome Binding Domain 1 (HMGN1) (AA 14-100) antibodyHMGN1 适用: 人 WB, ELISA, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Nucleosome Binding Domain 1 (HMGN1) (N-Term) antibodyHMGN1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Immunohistochemistry Dilution: 10 μg/mL
Application Note: Anti-IPO-38 has been tested by immunohistochemistry (frozen and formalin/paraffin) and western blot analysis on the Raji cell line and reacts with a 14 -16 kDa protein. This antibody is suitable for ELISA and immunoprecipitation. The antibody is reported to recognize a nuclear antigen that is present in the cytoplasm and nuclei of proliferating cells (paraformaldehyde fixed, Triton X-100 permeabilized). An increase of 400 % is observed in cells in mitosis (determined by analysis of K562 cells synchronized with colcemid). IPO-38 does not block the binding of the Ki-67 antibody. Tonsillar tissue is typically used as a positive control.
Western Blot Dilution: 1:500- 1:2,000
ELISA Dilution: 1:10,000 - 1:50,000
Other: User Optimized
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1.0 mg/mL
-
缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
-
有效期
- 12 months
-
-
- HMGN1 (High Mobility Group Nucleosome Binding Domain 1 (HMGN1))
-
别名
- Hmgn1
-
背景
- Background: The specificity of mAb IPO-38 was demonstrated in different human and murine lymphoid and non-lymphoid cell lines. It does not react with mononuclear cells and granulocytes of healthy donors but does react with nuclei of blood cells of patients with different forms of leukemia and with cells from lymph nodes of patients with Hodgkin's disease and non-hodgkin's lymphoma. Also a positive reaction in nuclei of breast and colorectal tumor cells was demonstrated. IPO-38 may be a good marker of cellular proliferation during monitoring of tumor progression.
-
基因ID
- 15312
-
NCBI登录号
- NP_032277
-
UniProt
- P18608
-
途径
- Chromatin Binding
抗原
-
