anti-Nuclear Factor I/A (NFIA) 抗体

NFIA encodes a member of the NF1 (nuclear factor 1) family of transcription factors. 再加上,我们可以发NFIA 蛋白 (8)NFIA 试剂盒 (2)和数多这个蛋白质的别的产品。

列出全部抗体 基因 基因ID UniProt
NFIA 4774 Q12857
NFIA 18027 Q02780
NFIA 25492 P09414
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Showing 10 out of 68 products:

产品编号 适用 宿主 标记 应用范围 图像 规格 交付 价格 详细
Cow 非结合性 WB WB Suggested Anti-NFIA Antibody Titration:  0.2-1 ug/ml  Positive Control:  Jurkat cell lysate Host:  Mouse  Target Name:  NFIA  Sample Tissue:  Mouse Testis  Antibody Dilution:  1ug/ml 100 μL 2至3个工作日
Cow 非结合性 WB WB Suggested Anti-Nfia Antibody   Titration: 1.0 ug/ml   Positive Control: Mouse Heart 100 μL 2至3个工作日
Cow 非结合性 WB Host:  Rabbit  Target Name:  Nfia  Sample Type:  Mouse Brain lysates  Antibody Dilution:  1.0ug/ml 100 μL 2至3个工作日
非结合性 IHC (p), WB Western blot analysis of NFIA using anti- NFIA antibody .  Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each  Lane was loaded with 50ug of sample under reducing conditions.   Lane 1: JURKAT whole cell lysates,   Lane 2: COLO320 whole cell lysates.  After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- NFIA antigen affinity purified polyclonal antibody (Catalog # ) at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFIA at approximately 69KD. The expected band size for NFIA is at 56KD. IHC analysis of NFIA using anti- NFIA antibody . NFIA was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti- NFIA Antibody  overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. 100 μg 4至6个工作日
小鸡 非结合性 IHC, WB Immunohistochemical analysis of NFIA staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Western blot analysis of NFIA expression in Jurkat (A), Raw264.7 (B), rat brain (C) whole cell lysates. 200 μL 13至14个工作日
Cow 非结合性 WB 100 μL 11至14个工作日
小鸡 非结合性 IHC, IHC (p), WB Human Brain, Cerebellum (formalin-fixed, paraffin-embedded) stained with NFIA antibody ABIN214127 at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody ABIN481713, alkaline phosphatase-streptavidin and chromogen. Human Prostate (formalin-fixed, paraffin-embedded) stained with NFIA antibody ABIN214127 at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody ABIN481713, alkaline phosphatase-streptavidin and chromogen. 100 μL 11至14个工作日
小鼠 非结合性 FACS, IHC (p), WB   100 μg 4至6个工作日
小鼠 非结合性 IF, ELISA, WB Detection limit for recombinant GST tagged NFIA is approximately 0.3ng/ml as a capture antibody. Immunofluorescence of monoclonal antibody to NFIA on HeLa cell. [antibody concentration 10 ug/ml] 100 μg 11至12个工作日
非结合性 IF, WB Western blot analysis in HepG2 cell line lysates (35ug/lane). Fluorescent confocal image of Hela cell stained with NFIA Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFIA primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NFIA immunoreactivity is localized to Nucleus significantly. 400 μL 2至3个工作日

引用最多的anti-NFIA 抗体

  1. Human Polyclonal NFIA Primary Antibody for WB - ABIN2668687 : Ravichandran, Sabath, Jensen, Houff, Major: Interactions between c-Jun, nuclear factor 1, and JC virus promoter sequences: implications for viral tropism. in Journal of virology 2006 (PubMed)
    Show all 5 Pubmed References

  2. Cow (Bovine) Polyclonal NFIA Primary Antibody for WB - ABIN6746156 : Foresti, Clark, Green, Motterlini: Thiol compounds interact with nitric oxide in regulating heme oxygenase-1 induction in endothelial cells. Involvement of superoxide and peroxynitrite anions. in The Journal of biological chemistry 1997 (PubMed)
    Show all 3 Pubmed References

  3. Human Monoclonal NFIA Primary Antibody for IF, ELISA - ABIN518309 : Rosa, Ballarino, Sorrentino, Sthandier, De Angelis, Marchioni, Masella, Guarini, Fatica, Peschle, Bozzoni: The interplay between the master transcription factor PU.1 and miR-424 regulates human monocyte/macrophage differentiation. in Proceedings of the National Academy of Sciences of the United States of America 2007 (PubMed)

  4. Human Polyclonal NFIA Primary Antibody for ICC, IF - ABIN4339318 : Bunt, Lim, Zhao, Mason, Richards: PAX6 does not regulate Nfia and Nfib expression during neocortical development. in Scientific reports 2015 (PubMed)


Human Nuclear Factor I/A (NFIA) interaction partners

  1. High Nuclear factor I A expresion is associated with temozolomide resistance in glioblastoma via activation of nuclear factor kappaB pathway.

  2. High NFIA expression in tumors of patients with esophageal squamous cell carcinoma (ESCC) is correlated with lymph node metastasis and poor differentiation. NFIA is found to be an independent predictor of poor prognosis in patients with ESCC.

  3. Transient expression of NFIA is sufficient to trigger glial competency of human pluripotent stem cell-derived neural stem cells within 5 days and to convert these cells into astrocytes in the presence of glial-promoting factors, as compared to 3-6 months using current protocols.

  4. The consistent overlap in clinical presentation provides further evidence of the critical role of NFIA haploinsufficiency in the development of the 1p32-p31 microdeletion syndrome phenotype.

  5. Data define a previously unknown nuclear factor I-A-nuclear factor-kappaB feed-forward regulation that may contribute to glioblastoma cell survival.

  6. Studies indicate the role of nuclear factor one (NFIs) as epigenetic regulators in cancer.

  7. Altogether, these results demonstrated that miR-370 suppressed hepatitis B virus gene expression and replication through repressing NFIA expression, which stimulates hepatitis B virus replication via direct regulation on hepatitis B virus Enhancer I activities.

  8. We verified that NFIA binds to the IGFBP2 promoter and transcriptionally enhances IGFBP2 expression levels. We identified that NFIA-mediated IGFBP2 signaling pathways are involved in miR-302b-induced glioma cell death.

  9. miR-191 was upregulated in patients with middle- and late-stage NSCLC, and in NSCLC cell lines, under mild hypoxic conditions. miR-191 promoted the proliferation and migration of NSCLC under chronic hypoxic conditions, and this promotion may be associated with its targeting of NFIA.

  10. Dihydrocapsaicin can significantly decrease proinflammatory cytokines through enhancing NFIA and inhibiting NF-kappaB expression

  11. These results demonstrated that RP5833A20.1 inhibited tumor cell proliferation, induced apoptosis and inhibited cellcycle progression by suppressing the expression of NFIA in U251 cells.

  12. NFI-A is involved in the miR-21-induced expression of IL-10 in B cells in nasopharyngeal carcinoma; Il-10 is capable of suppressing CD8+ T-cell activities.

  13. microRNA-136 targeted and degraded NFIA, which induced the release of microRNA-223, promoting CD11b expression. Direct base pairing occurs between miR-136 and the 3' UTR of NFIA mRNA.

  14. this family also carried a microdeletion affecting solely the NFIA gene, this study substantiates the importance of this gene in craniofacial development.

  15. TGF-beta-mediated suppression of ANT2 through NF1/Smad4 complexes contributes to oxidative stress and DNA damage during induction of cellular senescence.

  16. a strong candidate gene for asthma and allergic rhinitis

  17. RP5-833A20.1/miR-382-5p/NFIA pathway was essential to the regulation of cholesterol homeostasis and inflammatory reactions.

  18. This report presents the first case of an intragenic deletion within the NFIA gene that is still consistent with classic clinical phenotypes present in previously reported cases of chromosome 1p31.3 related deletion.

  19. High nuclear factor IA expression is associated with glioblastomas.

  20. These studies represent the first characterization of miR-223/NFIA axis function in glioma

Mouse (Murine) Nuclear Factor I/A (NFIA) interaction partners

  1. It identified NFIA and ATF3 as drivers of astrocyte differentiation from neural precursor cells while RUNX2 promotes astrocyte maturation.

  2. Our results support a myeloid cell loop in which NFI-A and miR-21 and miR-181b sustain Gr1(+)CD11b(+) MDSC-dependent immunosuppression during

  3. Of these, NFIA emerged as the regulator yielding the strongest molecular and cellular phenotypes. To examine its regulatory function, we profiled the genomic localization of NFIA, identifying it as a key early regulator of terminal brown fat cell differentiation.

  4. PAX6 can substitute for LHX2 and override NFIA-induced astrogliogenesis in developing hippocampus in vivo.

  5. The NFIA identifies a subset of bipolar cells as well as Muller glia and astrocytes. During early postnatal development, NFIA labels astrocytes on the day of birth, AII amacrine cells at postnatal (P) day 5, and Muller glia by P10, when horizontal cells also transiently exhibit NFIA immunofluorescence.

  6. NFIA expression regulates glioma tumorigenesis and glia-specific enhancers enhancers contribute to glioma formation.

  7. NFIA activates the cell-type-specific enhancers and facilitates the binding of PPARgamma to control the brown fat gene program.

  8. Dihydrocapsaicin can significantly decrease proinflammatory cytokines through enhancing NFIA and inhibiting NF-kappaB expression

  9. Astrocyte induction by Zbtb20 is suppressed by knockdown of NFIA.

  10. NFI-A disrupts myeloid cell differentiation and maturation in septic mice

  11. NFIA and NFIB are endogenously expressed in gradients with high caudo-medial to low rostro-lateral expression ... this expression pattern deviates from that of PAX6, suggesting that PAX6 does not drive Nfi expression

  12. RP5-833A20.1/miR-382-5p/NFIA pathway was essential to the regulation of cholesterol homeostasis and inflammatory reactions.

  13. Nfia cooperates with sonic hedgehog signaling to initiate medulloblastoma.

  14. Our data thus suggest that NFI nucleosomal interaction may contribute to the partitioning of distinct chromatin domains and to epigenetic gene expression regulation

  15. As maturing cerebellar granule neurons become more hyperpolarized, NFATc4 binding declines leading to onset of NFI temporal binding and the NFI switch program

  16. NFIA and NFIB are expressed in both the developing and the adult olfactory bulb indicative of a regulatory role for these transcription factors in the development of this facet of the olfactory system.

  17. NFIA is expressed in oligodendrocyte progenitors, but not differentiated oligodendrocytes during mouse embryonic development.

  18. NFIA is a transcription factor that plays a crucial role in the onset of gliogenesis; we found that its induction is regulated by the transcription factor Sox9 and that this relationship mediates the initiation of gliogenesis

  19. The studies demonstrate for the first time a requirement for the NFI-A transcription factor in antigen receptor-induced T-cell activation events.

  20. Nfia plays a central role in nervous system development, driving the expression of astrocyte-specific genes but also suppressing the progenitor cell state to allow differentiation to occur.

NFIA 抗原简介


This gene encodes a member of the NF1 (nuclear factor 1) family of transcription factors. Multiple transcript variants encoding different isoforms have been found for this gene.

Gene names and symbols associated with anti-Nuclear Factor I/A (NFIA) 抗体

  • nuclear factor I A (NFIA) 抗体
  • nuclear factor I/A (nfia) 抗体
  • nuclear factor I/A (Nfia) 抗体
  • 1110047K16Rik 抗体
  • 9430022M17Rik 抗体
  • CNFI-A 抗体
  • cNFI-A3 抗体
  • CTF 抗体
  • NF-I/A 抗体
  • NF1-A 抗体
  • NF1A 抗体
  • NFI-A 抗体
  • NFI-L 抗体
  • NFIA 抗体
  • si:ch211-88d2.2 抗体
  • wu:fq27e07 抗体
  • zgc:158351 抗体

Protein level used designations for anti-Nuclear Factor I/A (NFIA) 抗体

nuclear factor I/A , CCAAT-box-binding transcription factor , TGGCA-binding protein , nuclear factor 1 A-type , CTF , NF-I/A , NF1-A , NFI-A , nuclear factor 1/A , nuclear factor I-A

694022 Macaca mulatta
741619 Pan troglodytes
4774 Homo sapiens
556665 Danio rerio
396210 Gallus gallus
479552 Canis lupus familiaris
615371 Bos taurus
18027 Mus musculus
25492 Rattus norvegicus
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