GID Complex Subunit 8 Homolog (S. Cerevisiae) (GID8) (AA 1-228) protein (His tag)

ABIN3129238 产品详细信息, 供应商: Log in to see
蛋白名称
  • C20orf11
  • TWA1
  • Bwk1
  • 2310003C23Rik
  • C13H20orf11
  • GID complex subunit 8
  • GID complex subunit 8 homolog (S. cerevisiae)
  • GID8
  • Gid8
产品特性
AA 1-228
4
1
宿主
小鼠
4
1
资源
大肠杆菌(E. Coli)
2
1
1
1
蛋白类型
Recombinant
标记
This GID8 protein is labelled with His tag.
应用范围
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
选项
Supplier
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Supplier Product No.
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序列 MSYAEKPDEI TKDEWMEKLN NLHVQRADMN RLIMNYLVTE GFKEAAEKFR MESGIEPSVD LETLDERIKI REMILKGQIQ EAIALINSLH PELLDTNRYL YFHLQQQHLI ELIRQRETEA ALEFAQTQLA EQGEESRECL TEMERTLALL AFDSPEESPF GDLLHMMQRQ KVWSEVNQAV LDYENRESTP KLAKLLKLLL WAQNELDQKK VKYPKMTDLS KGVIEEPK
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
产品特性
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Mouse Gid8 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

纯化方法 Two step purification of proteins expressed in bacterial culture:
  1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
  2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
纯度 >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
过滤 0.22 μm filtered
内毒素水平 Endotoxin has not been removed. Please contact us if you require endotoxin removal.
质量等级 Crystallography grade
分子量 27.7 kDa Including tag.
UniProt Q9D7M1
应用备注 In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
说明

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

限制 仅限研究用
状态 Liquid
缓冲液 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
注意事项 Avoid repeated freeze-thaw cycles.
储存条件 -80 °C
储存方法 Store at -80°C.
有效期 Unlimited (if stored properly)
厂商提供的图像
 image for GID Complex Subunit 8 Homolog (S. Cerevisiae) (GID8) (AA 1-228) protein (His tag) (ABIN3129238) „Crystallography Grade“ protein due to multi-step, protein-specific purification process
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