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C-Reactive Protein (CRP) (AA 17-224) (Active) protein (His tag)

ABIN2487343 产品详细信息, 供应商: Log in to see
蛋白名称
  • crp
  • PTX1
  • AI255847
  • Aa1249
  • Ab1-341
  • Ab2-196
  • Ac1-114
  • Ac1262
  • Ac2-069
  • Ba2-693
  • APCS
  • 0610010I23Rik
  • AW743261
  • C77570
  • CRP2
  • CRP4
  • Crp
  • ESP1
  • Hlp
  • CRP
  • GRP-CB
  • C-reactive protein
  • C-reactive protein, pentraxin-related
  • cysteine rich protein 2
  • glutamine/glutamic acid-rich protein A
  • crp
  • CRP
  • Crp
  • Crip2
  • Grpcb
产品特性
AA 17-224
3
2
2
1
1
1
1
1
宿主

26
3
3
2
2
2
2
1
1
1
资源
HEK-293 Cells
21
12
3
3
1
蛋白类型
Recombinant
生物活性
Active
标记
His tag
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产品特性 This protein carries a polyhistidine tag at the C-terminus, and has a calculated MW of 24 kDa. The predicted N-terminus is Phe 17. The reducing (R) protein migrates as 26 kDa in SDS-PAGE. More than 90 % rhCRP exists as active homopentamer in analytical SEC-HPLC assay.
纯度 >95 % as determined by SDS-PAGE.
过滤 0.22 μm filtered
内毒素水平 Less than 1.0 EU per μg by the LAL method.
质量等级 HPLC verified
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA.Immobilized Human CRP/ C-reactive Protein (Cat# CRP-H5226) at 5μg/mL, can bind Biotinylated Human FcGR2A /CD32a Protein,Superior Sensitivity (Cat# CDA-H822R) with a linear range of 0.1-10 μg/mL.

SDS-PAGE:More than 90% rhCRP exists as active homopentamer in analytical SEC-HPLC assay.
背景 C-reactive protein (CRP) is a member of the pentraxin family of proteins that are characterized by a cyclic pentameric structure. Human CRP gene encodes a 224 amino acids precursor. The mature human CRP protein has 206 amino acids that are noncovalently linked to form the pentameter. Human CRP shares 71 % and 64 % amino acid sequence homology with mouse and rat respectively. CRP, synthesized by hepatocytes, is a major acute phase serum protein in human. IL6, IL1 and glucocorticoids are the major inducer of the CRP gene. The physiological role of CRP is to bind to phosphocholine expressed on the surface of dead or dying cells (and some types of bacteria) in order to activate the complement system. CRP binds to phosphocholine on microbes and damaged cells and enhances phagocytosis by macrophages. Thus, CRP participates in the clearance of necrotic and apoptotic cells. CRP rises up to 50,000-fold in acute inflammation, such as infection. It rises above normal limits within 6 hours, and peaks at 48 hours. Its half-life is constant, and therefore its level is mainly determined by the rate of production. It has been shown that high levels of CRP in humans is associated with an increased risk of cardiovascular diseases.
分子量 24 kDa
NCBI登录号 NP_000558
UniProt P02741
途径 Carbohydrate Homeostasis
限制 仅限研究用
状态 Lyophilized
溶解方式 Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
缓冲液 200 mM Tris, 1M NaCl, pH 8.0
注意事项 Avoid repeated freeze-thaw cycles.
储存条件 -20 °C
储存方法 No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
厂商提供的图像
SDS-PAGE (SDS) image for C-Reactive Protein (CRP) (AA 17-224) (Active) protein (His tag) (ABIN2487343) Human C-Reactive Protein, His Tag (HPLC-verified) on SDS-PAGE under reducing (R) cond...
有引用在: Meyer, Ueland: "Targeted quantification of C-reactive protein and cystatin c and its variants by immuno-MALDI-MS." in: Analytical chemistry, Vol. 86, Issue 12, pp. 5807-14, 2014 (PubMed).

背景 Lau, Dhillon, Yan, Szmitko, Verma: "Adipokines: molecular links between obesity and atheroslcerosis." in: American journal of physiology. Heart and circulatory physiology, Vol. 288, Issue 5, pp. H2031-41, 2005 (PubMed).