人 GSK3 alpha ELISA Kit

Recommended GSK3 alpha Product (supplied by: Log in to see )

抗原
Glycogen Synthase Kinase 3 alpha (GSK3a) ELISA试剂盒
  • GSK3A
  • CG5182
  • Dmel\\CG5182
  • GSK3a
  • 2700086H06Rik
  • xgsk3
  • GSK-3[a]
  • GSK-3[b]
  • GSK3
  • gsk3a
  • glycogen synthase kinase 3 alpha
  • CG5182 gene product from transcript CG5182-RA
  • glycogen synthase kinase 3 alpha b
  • GSK3A
  • gsk3a
  • Bm1_31380
  • Pk34A
  • Gsk3a
  • LOC100339192
  • gsk3ab
适用

其他选择
Kits with alternative reactivity to:
21
19
15
2
2
2
1
1
1
1
1
1
实验类型
Sandwich ELISA
检测范围
0.156-10 ng/mL
最低检测浓度
0.156 ng/mL
应用范围
ELISA
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产品编号 ABIN1570172
$ 832.83
加上运输费 $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
10.253036 ABIN416817 Sandwich ELISA Tissue Homogenate, Biological Fluids 0.15-10 ng/mL 0.15 ng/mL Log in to see
10.253036 ABIN1115208 Sandwich ELISA Serum, Plasma, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
10.253036 ABIN2039286 Serum, Plasma, Tissue Homogenate 0.312-20 ng/mL 0.312 ng/mL Log in to see
1 ABIN509681 Competition ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 0.5-10 ng/mL 0.5 ng/mL Log in to see
1 ABIN5654751 Sandwich ELISA Biological Fluids, Tissue Homogenate 0.156 ng/mL - 10 ng/mL 0.156 ng/mL Log in to see
1 ABIN1380592 Cell ELISA Log in to see
1 ABIN1381181 Cell ELISA Log in to see
1 ABIN4886117 Sandwich ELISA Cell Lysate, Tissue Lysate Log in to see
1 ABIN4881283 Cell ELISA Cell Culture Cells Log in to see
1 ABIN4886114 Sandwich ELISA Cell Lysate, Tissue Lysate Log in to see
1 ABIN5523850 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 0.156-10 ng/mL 0.156 ng/mL Log in to see
1 ABIN2113871 Cell ELISA Log in to see
1 ABIN2534018 Sandwich ELISA Tissue Homogenate, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
1 ABIN5596404 Sandwich ELISA Log in to see
1 ABIN5596403 Sandwich ELISA Log in to see
1 ABIN4969656 Sandwich ELISA Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum, Cell Lysate, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
1 ABIN2113870 Cell ELISA Log in to see
1 ABIN2684399 Cell ELISA Cell Samples, Suspension Cell Culture Log in to see
1 ABIN2947959 0.156-10 ng/mL 0.156 ng/mL Log in to see
1 ABIN1135202 Log in to see

General

抗原 Glycogen Synthase Kinase 3 alpha (GSK3a) ELISA试剂盒
适用
Kits with alternative reactivity to:
(21), (19), (15), (2), (2), (2), (1), (1), (1), (1), (1), (1)
实验类型 Sandwich ELISA
检测范围 0.156-10 ng/mL
最低检测浓度 0.156 ng/mL
应用范围 ELISA
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GSK3 alphaELISA Kit详情

目标详细情况 使用细节 贮存及处理 图像
原理 The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of GSK3a in human tissue homogenates and other biological fluids.
样品类型 Biological Fluids, Tissue Homogenate
检测方法 Colorimetric
Analytical Method Quantitative
特异性 This assay has high sensitivity and excellent specificity for detection of this index.
交叉反应 (详细) No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
灵敏度 0.049 ng/mL
组件
  • Pre-coated, ready to use 96-well strip plate
  • Standard (freeze dried)
  • Standard Diluent
  • Detection Reagent A
  • Detection Reagent B
  • Assay Diluent A
  • Assay Diluent B
  • TMB
  • Stop Solution
  • Wash Buffer (30X)
  • Plate sealer for 96 wells
  • Instruction manual
试剂未包括
  1. Microplate reader with 450 ± 10nm filter.
  2. Precision single or multi-channel pipettes and disposable tips.
  3. Eppendorf Tubes for diluting samples.
  4. Deionized or distilled water.
  5. Absorbent paper for blotting the microtiter plate.
  6. Container for Wash Solution.

目标详细情况

GSK3 alphaELISA Kit详情 使用细节 贮存及处理 图像 回页首
抗原
别名 GSK3a (GSK3a ELISA Kit 摘要)
基因ID 2931
UniProt P49840
途径 PI3K-Akt Signaling, WNT signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process

使用细节

GSK3 alphaELISA Kit详情 目标详细情况 贮存及处理 图像 回页首
样本量 100 μL
实验时间 1 - 4.5 h
板类型 Pre-coated
实验流程 1. Prepare all reagents, samples and standards
2. Add 100 µL standard or sample to each well. Incubate 2 hours at 37 °C
3. Aspirate and add 100 µL prepared Detection Reagent A. Incubate 1 hour at 37 °C
4. Aspirate and wash 3 times
5. Add 100 µL prepared Detection Reagent B. Incubate 30 minutes at 37 °C
6. Aspirate and wash 5 times
7. Add 90 µL Substrate Solution. Incubate 15-25 minutes at 37 °C
8. Add 50 µL Stop Solution. Read at 450nm immediately.
实验流程 The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.
实验精密度
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
  • CV(%) = SD/meanX100
  • Intra-assay: CV&lt10%
  • Inter-assay: CV&lt12%
限制 仅限研究用

贮存及处理

GSK3 alphaELISA Kit详情 目标详细情况 使用细节 图像 回页首
注意事项 The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
注意事项 The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
储存条件 4 °C,-20 °C
储存方法 The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
有效期 12 months

图像

GSK3 alphaELISA Kit详情 目标详细情况 使用细节 贮存及处理 回页首
厂商提供的图像
ELISA image for Glycogen Synthase Kinase 3 alpha (GSK3a) ELISA Kit (ABIN1570172) Glycogen Synthase Kinase 3 alpha (GSK3a) ELISA Kit