anti-人 RAF1 抗体 for ELISA

Recommended RAF1 Antibody (supplied by: Log in to see )

抗原
V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) 抗体
  • CRAF
  • NS5
  • Raf-1
  • c-Raf
  • c-raf
  • craf
  • raf1
  • RAF1
  • 6430402F14Rik
  • AA990557
  • BB129353
  • Craf1
  • D830050J10Rik
  • v-Raf
  • raf
  • cRaf
  • Raf-1 proto-oncogene, serine/threonine kinase
  • Raf-1 proto-oncogene, serine/threonine kinase a
  • RAF proto-oncogene serine/threonine-protein kinase pseudogene
  • v-raf-leukemia viral oncogene 1
  • Raf-1 proto-oncogene, serine/threonine kinase S homeolog
  • RAF1
  • Raf1
  • raf1a
  • raf1
  • LOC100341921
  • raf1.S
适用

831
409
386
25
23
16
16
13
8
8
8
5
4
4
2
2
1
1
1
宿主
小鼠
777
62
12
6
2
克隆类型
单克隆
标记
This RAF1 antibody is un-conjugated
45
45
44
30
29
29
14
14
12
12
12
12
12
12
12
12
12
2
2
2
2
2
2
2
2
2
1
1
应用范围
Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
508
364
217
172
156
104
52
48
20
18
18
7
2
2
2
1
选项
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产品编号 ABIN969560
$ 430.10
加上运输费 $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
10.138596 ABIN969559 FACS IHC ELISA WB Mouse IgG1 Log in to see 4G4 2
10.138596 ABIN4349225 CyTOF ELISA FACS IHC WB Mouse IgG1 Log in to see 4G4
10.138596 ABIN1846392 FACS IHC ELISA WB Mouse IgG1 Log in to see
10.138596 ABIN1844891 FACS IHC ELISA WB Mouse IgG1 Log in to see
10.138596 ABIN1847644 IHC ELISA Rabbit Internal Region, pThr269 Log in to see Polyclonal
1 ABIN1532586 IHC ELISA WB Rabbit IgG AA 11-60 Log in to see Polyclonal 1
1 ABIN1532076 IHC ELISA Rabbit IgG AA 231-280, pThr269 Log in to see Polyclonal 1
1 ABIN1532992 IHC ELISA WB Rabbit IgG AA 225-274 Log in to see Polyclonal 1
1 ABIN1533109 IHC ELISA Rabbit IgG AA 231-280 Log in to see Polyclonal 1
1 ABIN1532077 IHC ELISA Rabbit IgG AA 251-300, pSer289 Log in to see Polyclonal 1
1 ABIN1531571 ELISA WB Rabbit IgG AA 11-60, pSer43 Log in to see Polyclonal 2
1 ABIN1531958 IHC ELISA WB Rabbit IgG AA 225-274 Log in to see Polyclonal 1
1 ABIN1533110 IHC ELISA WB Rabbit IgG AA 251-300 Log in to see Polyclonal
1 ABIN1532444 IHC ELISA WB Rabbit IgG AA 271-320 Log in to see Polyclonal
1 ABIN1532404 ELISA WB Rabbit IgG AA 311-360 Log in to see Polyclonal
1 ABIN1532405 IF IHC ELISA WB Rabbit IgG AA 591-640 Log in to see Polyclonal
1 ABIN1531428 IHC ELISA WB Rabbit IgG AA 271-320, pSer296 Log in to see Polyclonal
1 ABIN1531959 ELISA WB Rabbit IgG AA 305-354, pSer338 Log in to see Polyclonal
1 ABIN1532993 ELISA WB Rabbit IgG AA 305-354 Log in to see Polyclonal
1 ABIN1531385 IHC ELISA WB Rabbit IgG AA 311-360, pTyr341 Log in to see Polyclonal

General

抗原 V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) 抗体
适用
(831), (409), (386), (25), (23), (16), (16), (13), (8), (8), (8), (5), (4), (4), (2), (2), (1), (1), (1)
宿主 小鼠
(777), (62), (12), (6), (2)
克隆类型 (克隆位点)
单克隆   ( )
标记 This RAF1 antibody is un-conjugated
(45), (45), (44), (30), (29), (29), (14), (14), (12), (12), (12), (12), (12), (12), (12), (12), (12), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
应用范围 Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(508), (364), (217), (172), (156), (104), (52), (48), (20), (18), (18), (7), (2), (2), (2), (1)
Pubmed 2个引用可参考
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RAF1抗体详情

RAF1目标详情 使用细节 贮存及处理 References for anti-RAF1 antibody (ABIN969560) 图像
纯化方法 Purified
免疫原 Purified recombinant fragment of human RAF1 expressed in E. Coli.
克隆位点 4G4
亚型 IgG1

RAF1目标详情

RAF1抗体详情 使用细节 贮存及处理 References for anti-RAF1 antibody (ABIN969560) 图像 回页首
抗原
别名 RAF1 (RAF1 Antibody 摘要)
背景 This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
分子量 73 kDa
基因ID 5894
HGNC 5894
研究领域 Signaling, Kinases/Phosphatases
途径 MAPK Pathway, RTK signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling

使用细节

RAF1抗体详情 RAF1目标详情 贮存及处理 References for anti-RAF1 antibody (ABIN969560) 图像 回页首
应用备注 ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, FCM: 1:200 - 1:400
限制 仅限研究用

贮存及处理

RAF1抗体详情 RAF1目标详情 使用细节 References for anti-RAF1 antibody (ABIN969560) 图像 回页首
状态 Liquid
缓冲液 PBS with 0.05 % sodium azide
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C,-20 °C
储存方法 4°C, -20°C for long term storage

References for anti-RAF1 antibody (ABIN969560)

RAF1抗体详情 RAF1目标详情 使用细节 贮存及处理 图像 回页首
有引用在:

Takezawa, Okamoto, Yonesaka, Hatashita, Yamada, Fukuoka, Nakagawa: "Sorafenib inhibits non-small cell lung cancer cell growth by targeting B-RAF in KRAS wild-type cells and C-RAF in KRAS mutant cells." in: Cancer research, Vol. 69, Issue 16, pp. 6515-21, 2009

Peng, Guo, Ji, Ren, Yang, Li, Qu, Li, Tang, Yan, Tang: "Sorafenib induces growth inhibition and apoptosis in human synovial sarcoma cells via inhibiting the RAF/MEK/ERK signaling pathway." in: Cancer biology & therapy, Vol. 8, Issue 18, pp. 1729-36, 2009

图像

RAF1抗体详情 RAF1目标详情 使用细节 贮存及处理 References for anti-RAF1 antibody (ABIN969560) 回页首
厂商提供的图像
ELISA image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) antibody (ABIN969560) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) antibody (ABIN969560) Figure 2: Flow cytometric analysis of MCF-7 cells using RAF1 mouse mAb (green) and ne...
Immunohistochemistry (IHC) image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) antibody (ABIN969560) Figure 3: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues us...
Western Blotting (WB) image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) antibody (ABIN969560) Figure 4: Western blot analysis using RAF1 mouse mAb against HeLa (1), A431 (2), HepG...
Western Blotting (WB) image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) antibody (ABIN969560) Figure 5: Western blot analysis using RAF1 mAb against human RAF1 (AA: 198-407) recom...