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dsDNA Ab ELISA 试剂盒

dsDNA Ab 适用: 人 Colorimetric Competition ELISA Serum
产品编号 ABIN996874
发货至: 中国
  • 抗原 See all dsDNA Ab products
    dsDNA Ab (Anti-Double Strand DNA Antibody (dsDNA Ab))
    适用
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    检测方法
    Colorimetric
    实验类型
    Competition ELISA
    应用范围
    ELISA
    原理
    dsDNA ELISA is intended for the detection of antibodies in human serum to dsDNA antigen and as an aid in diagnosis of Systemic lupus eruthematosus (SLE).
    样品类型
    Serum
    Analytical Method
    Semi-Quantitative
    特异性
    97.9 %
    灵敏度
    100%
    组件
    1. Microplate: wells coated with Purified dsDNA antigen
    2. Serum Diluent Type II
    3. Positive Control: Human serum or defibrinated plasma
    4. Calibrator: Human serum or defibrinated plasma
    5. Negative Control: Human serum or defibrinated plasma
    6. HRP Conjugate: Goat anti-human IgG and IgM
    7. Wash Buffer Type I 20X
    8. Chromogen/Substrate Solution Type I
    9. Stop Solution: 1N H2SO4 solution
    试剂未包括
    1. Precision pipettes
    2. Distilled or deionized water
    3. EIA kit Microplate Washer
    4. EIA kit Microplate Reader with a (single wavelength 450 nm or dual 600-650)
  • 应用备注
    Notable features of Autoimmune Disease ELISA Kits:
    - User-friendly directions and explanation of test procedures
    - Simple and safe reagent preparation
    - Clear instructions on specimen collection
    - Comprehensive package of required materials
    - Explicit quality control and storage guidelines
    - Reliable and easy-to-read test results
    样本量
    10 μL
    实验时间
    1.5 h
    板类型
    Pre-coated
    实验流程
    dsDNA elisa test is an Enzyme-Linked Immunosorbent Assay to detect antibodies to dsDNA antigen. Purified dsDNA antigen is attached to the wells. When antigens bound to the solid phase are brought into contact with a patient serum, antigen specific antibody, if present, will bind to the antigen in the wells forming antigen-antibody complexes. Excess antibody is removed by washing and followed by the addition of goat anti-human IgG, M conjugated with horseradish peroxidase which then binds to the antibody-antigen complexes.
    限制
    仅限研究用
  • 储存条件
    4 °C
    有效期
    12-14 months
  • Salazar, Copeland, Wood, Schmid, Luebke: "Evaluation of anti-nuclear antibodies and kidney pathology in Lewis rats following exposure to Libby amphibole asbestos." in: Journal of immunotoxicology, (2012) (PubMed).

  • 抗原 See all dsDNA Ab products
    dsDNA Ab (Anti-Double Strand DNA Antibody (dsDNA Ab))
    别名
    DsDNA Antibody (dsDNA Ab 产品)
    物质类
    Antibody, Antibody
    背景
    Autoimmune diseases are categorized into two general groups: One group is characterized by the production of tissue-specific autoantibodies, the other by autoimmune reactivity with normal cell nuclear and/or cytoplasmic antigens with no tissue type specificity. The latter group includes diseases such as mixed connective tissue disease, scleroderma, rheumatoid arthritis, dermatomyositis, polymyositis, SLE and Sjogren syndrome.
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