Rabbit Peroxidase Anti-Peroxidase (PAP) Soluble Complex

ABIN965456 产品详细信息, 供应商: Log in to see
标记
HRP
应用范围
Labeling (Lbl)
选项
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免疫原 Peroxidase (Horseradish)
特异性 This product was prepared from rabbit polyclonal antiserum by delipidation, salt fractionation and ion exchange chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and anti-Peroxidase.
背景 The peroxidase-antiperoxidase (PAP) approach utilizes a complex of enzyme and antibody to facilitate amplification of signal, indirectly detecting the amount of primary antibody used in an assay. Generated in Rabbit, the PAP complex can be utilized in any assay that utilizes a rabbit primary antibody. With the addition of a secondary antibody that recognizes rabbit, the reporter PAP complex allows for amplification of the luminescent signal (as three molecules of enzyme are bound to every two immunoglobulins).
Synonyms: Horseradish peroxidase; HRP
应用备注 Suitable for use in peroxidase enzymatic labeling experiments.
说明

Detection Kit Type: PAP Kit

限制 仅限研究用
状态 Lyophilized
溶解方式 Reconstitution Buffer: Restore with PBS + 5% normal bridging host serum
浓度 5.0 mg/mL. Concentration Definition: by UV absorbance at 280 nm
缓冲液 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2.
储存条件 4 °C