GLDC ELISA 试剂盒
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- 抗原 See all GLDC ELISA试剂盒
- GLDC (Glycine Dehydrogenase (GLDC))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.312 ng/mL - 20 ng/mL
- 最低检测浓度
- 0.312 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Glycine Dehydrogenase (GLDC). No significant cross-reactivity or interference between Glycine Dehydrogenase (GLDC) and analogues was observed.
- 灵敏度
- 0.132 ng/mL
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Glycine Dehydrogenase (GLDC) ELISA Kit
GLDC 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Glycine Dehydrogenase (GLDC) ELISA KitGLDC 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Glycine Dehydrogenase (GLDC) ELISA KitGLDC 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Glycine Dehydrogenase (GLDC) ELISA KitGLDC 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycine Dehydrogenase (GLDC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glycine Dehydrogenase (GLDC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycine Dehydrogenase (GLDC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glycine Dehydrogenase (GLDC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glycine Dehydrogenase (GLDC) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glycine Dehydrogenase (GLDC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all GLDC ELISA试剂盒
- GLDC (Glycine Dehydrogenase (GLDC))
- 别名
- Glycine Dehydrogenase (GLDC 产品)
- 别名
- PSPTO1276 ELISA Kit, Tb07.43M14.350 ELISA Kit, DDBDRAFT_0219205 ELISA Kit, DDBDRAFT_0231130 ELISA Kit, DDB_0219205 ELISA Kit, DDB_0231130 ELISA Kit, D030049L12Rik ELISA Kit, D19Wsu57e ELISA Kit, GCE ELISA Kit, GCSP ELISA Kit, HYGN1 ELISA Kit, fb23b05 ELISA Kit, wu:fb23b05 ELISA Kit, zgc:66198 ELISA Kit, gldc ELISA Kit, glycine dehydrogenase (decarboxylating) GcvP ELISA Kit, glycine dehydrogenase ELISA Kit, glycine cleavage system P-protein ELISA Kit, glycine decarboxylase ELISA Kit, glycine dehydrogenase (decarboxylating) ELISA Kit, glycine decarboxylase S homeolog ELISA Kit, gcvP ELISA Kit, Tb927.7.1910 ELISA Kit, Gldc ELISA Kit, GLDC ELISA Kit, gldc ELISA Kit, gldc.S ELISA Kit
- 背景
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Gene Name: Glycine Dehydrogenase
Gene Aliases: GCSP, NKH, Decarboxylating, Glycine Decarboxylase, Glycine Cleavage System Protein P, Glycine dehydrogenase (aminomethyl-transferring)
- 基因ID
- 2731
- UniProt
- P23378
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