Follistatin ELISA 试剂盒

• FSTL-3 Calibrator A/Sample Diluent
• FSTL-3 Calibrators B thru F (Lyophilized)
• FSTL-3 Controls I & II (Lyophilized)
• FSTL-3 Antibody Coated Microtiter plate Strips
• FSTL-3 Assay Buffer
• FSTL-3 Biotin Conjugate Concentrate
• FSTL-3 Biotin Conjugate Diluent
• FSTL-3 Streptavidin Enzyme Conjugate Ready To Use
• TMB Chromogen Solution
• Stopping Solution
• Wash Concentrate A

1. Microplate absorbance reader capable of absorbance measurement at 450 nm, 405nm,and 630 nm.
2. Microplate orbital shaker.
3. Microplate washer.
4. Vortex mixer.
5. Repeater pipette.

1. Wash Solution: Dilute wash concentrate 25-fold with deionized water. The wash solution is stable for one month at room temperature when stored in a tightly sealed bottle.
2. Microtitration Wells: Select the number of coated wells required for the assay. The remaining unused wells should be placed in the resealable pouch with a desiccant. The pouch must be resealed to protect from moisture.
3. Estriol Biotin Conjugate Solution: The Estriol Biotin Conjugate Concentrate should be diluted at a ratio of 1 part conjugate to 50 parts of Estriol Conjugate Diluent, according to the number of wells used. If an entire plate is to be used pipet exactly 220 μL of the Concentrate into 11 mL of the diluent. Estriol biotin conjugate concentrate should be freshly diluted 10 minutes prior to use.

• Serum is the recommended sample type.
• Sample handling, processing, and storage requirements depend on the brand of blood collection tube that you use. Please reference the manufacturer's instructions for guidance. Each laboratory should determine the acceptability of its own blood collection tubes and serum separation products.
• Samples may be stored at 4 °C if assayed within 24 hours, otherwise samples must be stored at -20 °C or -80 °C to avoid loss of bioactivity and contamination.
• Avoid assaying lipemic, hemolyzed or icteric samples.
• Avoid repeated freezing and thawing of samples. Thaw samples no more than 3 times.
• For shipping, place specimens in leak proof containers in biohazard specimen bags with appropriate specimen identification and test requisition information in the outside pocket of the biohazard specimen bag. Follow DOT and IATA requirements when shipping specimens.

Allow all specimens and reagents to reach room temperature (23 ± 2 °C) and mix thoroughly by gentle inversion before use. Calibrators, controls, and unknowns should be assayed in duplicate. Note: All samples should be diluted 1:5 (20 μL sample+ 80 μL FSTL-3 Calibrator A/Sample Diluent). Do not dilute the calibrators and controls.

1. Allow the calibrators to reach the room temperature (23 ± 2 °C) & mix well by gentle vortex.
2. Label the microtiter strips to be used.
3. Pipette 25 μL of the Calibrator, Controls and Unknowns to the appropriate wells.
4. Add 100 μL of the FSTL-3 Assay Buffer to each well using a repeater pipette.
5. Incubate the plate, shaking at a fast speed (600-800 rpm) on an orbital microplate shaker, for 60 minutes at room temperature (23 ± 2 °C).
6. With 30-40 minutes remaining of incubation time, prepare the FSTL-3 Antibody-Biotin Conjugate Solution by diluting the FSTL-3 Biotin Conjugate Concentrate in FSTL-3 Conjugate Diluent as described under the Preparation of the Reagents section of this insert.
7. Aspirate and wash each strip 5 times with Wash Solution (350 μL/per well) using an automatic microplate washer.
8. Add 100 μL of the FSTL-3 Biotin Conjugate solution to each well using a repeater pipette.
9. Incubate the plate, shaking at a fast speed (600-800 rpm) on an orbital microplate shaker, for 60 minutes at room temperature (23 ± 2 °C).
10. Aspirate and wash each strip 5 times with the Wash Solution (350 μL/per well) using an automatic microplate washer.

NOTE: The results in this package insert were calculated by plotting the data on a log vs. log scale using a cubic regression curve-fit. Other data reduction methods may give slightly different results.

1. Calculate the mean optical density (OD) for each calibrator, Control, or Unknown.
2. Plot the log of the mean OD readings for each of the Calibrators along the y-axis versus log of the FSTL-3 concentrations in ng/mL along the x-axis, using a cubic regression curve-fit.
3. Determine the FSTL-3 concentrations of the Controls and unknowns from FSTL-3 concentrations.
4. Any sample reading higher than the highest Calibrator should be appropriately diluted with the 0 ng/mL (CAL A / Sample Diluent) and re- assayed.
5. Any sample reading lower than the analytical sensitivity should be reported as such.
6. Multiply the value by a dilution factor.