MST1 ELISA 试剂盒 (Macrophage Stimulating 1 (Hepatocyte Growth Factor-Like))

Details for Product MST1 ELISA Kit No. ABIN1889321, 供应商: Log in to see
抗原
  • D3F15S2
  • DNF15S2
  • HGFL
  • MSP
  • NF15S2
  • D3F15S2h
  • D9H3F15S2
  • DNF15S2h
  • Hgfl
  • E2F2
  • HGF1/MSP
  • macrophage stimulating 1 (hepatocyte growth factor-like)
  • Macrophage stimulating 1 (hepatocyte growth factor-like)
  • MST1
  • Mst1
抗原表位
AA 19-711
适用

其他选择
Kits with alternative reactivity to:
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11
5
2
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2
2
1
1
1
实验类型
Sandwich ELISA
检测范围
156-10000 pg/mL
最低检测浓度
156 pg/mL
应用范围
ELISA
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原理 Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human MSP/MST1
品牌 PicoKine™
样品类型 Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Analytical Method Quantitative
检测方法 Colorimetric
免疫原 Expression system for standard: NSO
Immunogen sequence: Q19-G711
特异性 Expression system for standard: NSO
Immunogen sequence: Q19-G711
交叉反应 (详细) There is no detectable cross-reactivity with other relevant proteins.
灵敏度 <10pg/mL
试剂未包括 Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
别名 MST1 (MST1 ELISA Kit 摘要)
背景

Background: Macrophage Stimulating 1(SMT1), also known as Hepatocyte growth factor-like protein(HGFL) and MSP, is a protein that in humans is encoded by the MST1 gene. The HGFL gene was identified at the DNF15S2 locus on human chromosome 3(3p21). The RON tyrosine kinase), the receptor for MSP, is expressed on the ciliated epithelia of the mucociliary transport apparatus of the lung. Furthermore, MSP stimulated ciliary motility in these cells by activating RON.

Synonyms: Hepatocyte growth factor-like protein,Macrophage stimulatory protein,Macrophage-stimulating protein,MSP,Hepatocyte growth factor-like protein alpha chain,Hepatocyte growth factor-like protein beta chain,MST1,D3F15S2, DNF15S2, HGFL,

Full Gene Name: Hepatocyte growth factor-like protein

Cellular Localisation: Secreted.
基因ID 4485
UniProt P26927
研究领域 Cancer, Innate Immunity, Signaling
应用备注 Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
说明

Sequence similarities: Belongs to the peptidase S1 family. Plasminogen subfamily.

板类型 Pre-coated
实验流程 human MSP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MSP has been precoated onto 96-well plates. Standards(NSO, Q19-G711) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MSP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MSP amount of sample captured in plate.
实验流程

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human MSP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human MSP standard solution and each sample be measured in duplicate.

实验精密度
  • Sample 1: n=16, Mean(pg/ml): 1126, Standard deviation: 56.3, CV(%): 5
  • Sample 2: n=16, Mean(pg/ml): 2738, Standard deviation: 170, CV(%): 6.2
  • Sample 3: n=16, Mean(pg/ml): 5273, Standard deviation: 242.6, CV(%): 4.6,
  • Sample 1: n=24, Mean(pg/ml): 1384, Standard deviation: 78.9, CV(%): 5.7
  • Sample 2: n=24, Mean(pg/ml): 2941, Standard deviation: 206, CV(%): 7
  • Sample 3: n=24, Mean(pg/ml): 5527, Standard deviation: 364.8, CV(%): 6.6
限制 仅限研究用
注意事项 Avoid multiple freeze-thaw cycles.
储存条件 -20 °C,4 °C
储存方法 Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
有效期 12 months
厂商提供的图像
ELISA image for MST1 ELISA 试剂盒 (Macrophage Stimulating 1 (Hepatocyte Growth Factor-Like)) (ABIN1889321) Human MSP/MST1 PicoKine ELISA Kit standard curve
背景 Sakamoto, Iwama, Amitani, Takehara, Yamaguchi, Yamamoto, Masuyama, Yamanaka, Ando, Suda: "Role of macrophage-stimulating protein and its receptor, RON tyrosine kinase, in ciliary motility." in: The Journal of clinical investigation, Vol. 99, Issue 4, pp. 701-9, 1997 (PubMed).

Han, Stuart, Degen: "Characterization of the DNF15S2 locus on human chromosome 3: identification of a gene coding for four kringle domains with homology to hepatocyte growth factor." in: Biochemistry, Vol. 30, Issue 40, pp. 9768-80, 1991 (PubMed).

Gerber, Drabkin, Firnhaber, Miller, Scoggin, Smith: "Regional localization of chromosome 3-specific DNA fragments by using a hybrid cell deletion mapping panel." in: American journal of human genetics, Vol. 43, Issue 4, pp. 442-51, 1988 (PubMed).