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CMV IgG ELISA 试剂盒

CMV IgG 适用: 人 Colorimetric Competition ELISA Plasma, Serum
产品编号 ABIN1326812
发货至: 中国
  • 抗原 See all CMV IgG products
    CMV IgG (Anti-Cytomegalovirus IgG Antibody (CMV IgG))
    适用
    检测方法
    Colorimetric
    实验类型
    Competition ELISA
    应用范围
    ELISA
    原理
    CMV IgG ELISA Kit is intended for the detection of IgG antibody to Cytomegalovirus (CMV) in human serum or plasma.
    样品类型
    Plasma, Serum
    Analytical Method
    Qualitative
  • 应用备注
    Diluted patient serum is added to wells coated with purified antigen. IgG specific antibody, if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibody-antigen complex, if present. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of IgG specific antibody in the sample.
    板类型
    Pre-coated
    结果分析
    • Check Calibrator Factor (CF) value on the calibrator bottle. This value might vary from lot to lot. Make sure you check the value on every kit.
    • Calculate the cut-off value: Calibrator OD x Calibrator Factor (CF).
    • Calculate the Ab (Antibody) Index of each determination by dividing the O.D. value of each sample by cut-off value.
    限制
    仅限研究用
  • 储存条件
    4 °C
  • Wang, Liu, Xu, Jackson, Roskin, Pham, Laserson, Marshall, Seo, Lee, Furman, Koller, Dekker, Davis, Fire, Boyd: "Effects of aging, cytomegalovirus infection, and EBV infection on human B cell repertoires." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 192, Issue 2, pp. 603-11, (2014) (PubMed).

  • 抗原 See all CMV IgG products
    CMV IgG (Anti-Cytomegalovirus IgG Antibody (CMV IgG))
    别名
    CMV IgG (CMV IgG 产品)
    物质类
    Antibody
    背景
    Cytomegalovirus (CMV) is a member of the herpes group of viruses. Most adults and children who catch CMV have no symptoms and are not harmed by the virus. CMV infection is of clinical significance primarily in pregnant women, newborn infants with possible congenital infection, immunosuppressed transplant patients and iniduals with AIDS. CMV is so prevalent as over 60% of people catch the infection at some time in their lives.Significant increases in CMV IgG antibody by ELISA suggest recent infection or reactivation of a latent CMV infection.ELISA can detect CMV IgM antibody in both primary CMV infections (93-100%) and in reactivated infection (40%). An IgM response may be reduced or absent in immunocompromised patients with active infection. In transplant patients the CMV infection can be associated with higher morbidity and mortality.
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