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PSIP1 抗体 (AA 85-188)

PSIP1 适用: 人 WB, IF, BI 宿主: 小鼠 Monoclonal 26-LEDGF unconjugated
产品编号 ABIN968640
发货至: 中国
  • 抗原 See all PSIP1 抗体
    PSIP1 (PC4 and SFRS1 Interacting Protein 1 (PSIP1))
    抗原表位
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 85-188
    适用
    • 26
    • 19
    • 12
    • 12
    • 6
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    宿主
    • 16
    • 13
    小鼠
    克隆类型
    • 16
    • 13
    单克隆
    标记
    • 22
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This PSIP1 antibody is un-conjugated
    应用范围
    • 19
    • 9
    • 8
    • 4
    • 3
    • 3
    • 3
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), BioImaging (BI)
    产品特性
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    纯化方法
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    免疫原
    Human LEDGF aa. 85-188
    克隆位点
    26-LEDGF
    亚型
    IgG1
    Top Product
    Discover our top product PSIP1 Primary Antibody
  • 应用备注
    Bioimaging:
    Methanol Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 90% methanol. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Wash three times with PBS. Flick out PBS and add second step reagent. Incubate for 1 hour at RT. Wash three times with PBS.
    Triton-X 100 Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 0.1% Triton-X 100. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Flick out and wash three times with PBS. Flick out and add second step reagent. Incubate for 1 hour at RT. Flick out and wash three times with PBS.
    说明

    Related Products: ABIN968535, ABIN967389

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    250 μg/mL
    缓冲液
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
    储存方法
    Store undiluted at -20°C.
  • Cherepanov, Maertens, Proost, Devreese, Van Beeumen, Engelborghs, De Clercq, Debyser: "HIV-1 integrase forms stable tetramers and associates with LEDGF/p75 protein in human cells." in: The Journal of biological chemistry, Vol. 278, Issue 1, pp. 372-81, (2002) (PubMed).

    Nakamura, Singh, Kubo, Chylack, Shinohara: "LEDGF: survival of embryonic chick retinal photoreceptor cells." in: Investigative ophthalmology & visual science, Vol. 41, Issue 5, pp. 1168-75, (2000) (PubMed).

    Singh, Kimura, Chylack, Shinohara: "Lens epithelium-derived growth factor (LEDGF/p75) and p52 are derived from a single gene by alternative splicing." in: Gene, Vol. 242, Issue 1-2, pp. 265-73, (2000) (PubMed).

    Ge, Si, Wolffe: "A novel transcriptional coactivator, p52, functionally interacts with the essential splicing factor ASF/SF2." in: Molecular cell, Vol. 2, Issue 6, pp. 751-9, (1999) (PubMed).

    Ge, Si, Roeder: "Isolation of cDNAs encoding novel transcription coactivators p52 and p75 reveals an alternate regulatory mechanism of transcriptional activation." in: The EMBO journal, Vol. 17, Issue 22, pp. 6723-9, (1999) (PubMed).

  • 抗原
    PSIP1 (PC4 and SFRS1 Interacting Protein 1 (PSIP1))
    别名
    LEDGF (PSIP1 产品)
    别名
    PSIP1 antibody, im:7149861 antibody, zgc:109898 antibody, LEDGF antibody, p75 antibody, PSIP2 antibody, DFS70 antibody, PAIP antibody, p52 antibody, AA408851 antibody, AU015605 antibody, Dfs70 antibody, Ledgfa antibody, Ledgfb antibody, Psip2 antibody, Ledgf antibody, SBP75 antibody, LEDGF/p75 antibody, PC4 and SFRS1 interacting protein 1 S homeolog antibody, PC4 and SFRS1 interacting protein 1 antibody, PC4 and SFRS1 interacting protein 1a antibody, LEDGF/p75 transcription factor-like antibody, psip1.S antibody, PSIP1 antibody, psip1a antibody, psip1 antibody, Psip1 antibody
    背景
    P52/p75 proteins were first identified through their interaction with transcriptional factors and splicing factors. p75 is encoded by exons 1-15 and contains 530 amino acids, while the alternatively spliced p52 is encoded by exons 1-9 and contains 333 amino acids. Both p52 and p75 have strong interactions with the VP16 activation domain and with various components of the general transcriptional machinery. p52 is the more active transcription activator and has also been shown to recruit the pre-mRNA splicing factor ASF/SF to sites of active transcription. Interestingly, p52/p75 has also been identified as Lens Epithelium-Derived Growth Factor (LEDGF). In serum free media, LEDGF stimulates the growth and survival of a variety of cells, such as lens epithelial cells, retinal photoreceptor cells, COS7 cells, skin fibroblasts, and keratinocytes. In retinal photoreceptor cells, LEDGF is found in the nucleus where it promotes resistance to serum starvation and thermal stress. Thus, the LEDGF gene encodes splice variants that may regulate gene transcription and mRNA splicing of a variety of proteins that are essential for cell growth and survival.
    Synonyms: Lens Epithelium-Derived Growth Factor
    分子量
    75 kDa and/or 52 kDa
    途径
    Chromatin Binding, Ribonucleoprotein Complex Subunit Organization
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