PIK3C2B 抗体 (AA 16-209)
(1 reference)-
- 抗原 See all PIK3C2B 抗体
- PIK3C2B (Phosphatidylinositol-4-Phosphate 3-Kinase, Catalytic Subunit Type 2 beta (PIK3C2B))
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抗原表位
- AA 16-209
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This PIK3C2B antibody is un-conjugated
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应用范围
- Western Blotting (WB), BioImaging (BI)
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
6. Triton is a trademark of the Dow Chemical Company. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Human PI3-Kinase C2beta aa. 16-209
- 克隆位点
- 22-PI3
- 亚型
- IgG1
- Top Product
- Discover our top product PIK3C2B Primary Antibody
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anti-Phosphatidylinositol-4-Phosphate 3-Kinase, Catalytic Subunit Type 2 beta (PIK3C2B) antibody
PIK3C2B 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 3B1 unconjugated
anti-Phosphatidylinositol-4-Phosphate 3-Kinase, Catalytic Subunit Type 2 beta (PIK3C2B) (AA 1-110) antibodyPIK3C2B 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1H4 unconjugated
anti-Phosphatidylinositol-4-Phosphate 3-Kinase, Catalytic Subunit Type 2 beta (PIK3C2B) antibodyPIK3C2B 适用: 人 WB, IHC, IHC (p), IF 宿主: 小鼠 Monoclonal 2G3 unconjugated
anti-Phosphatidylinositol-4-Phosphate 3-Kinase, Catalytic Subunit Type 2 beta (PIK3C2B) (AA 790-1025) antibodyPIK3C2B 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Phosphatidylinositol-4-Phosphate 3-Kinase, Catalytic Subunit Type 2 beta (PIK3C2B) (AA 1-110) antibodyPIK3C2B 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3E5 unconjugated
anti-Phosphatidylinositol-4-Phosphate 3-Kinase, Catalytic Subunit Type 2 beta (PIK3C2B) (Internal Region) antibodyPIK3C2B 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Phosphatidylinositol-4-Phosphate 3-Kinase, Catalytic Subunit Type 2 beta (PIK3C2B) (AA 215-250), (pTyr228) antibodyPIK3C2B 适用: 人 WB 宿主: 兔 Polyclonal RB49201 unconjugated
anti-Phosphatidylinositol-4-Phosphate 3-Kinase, Catalytic Subunit Type 2 beta (PIK3C2B) (AA 1-110) antibodyPIK3C2B 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
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- 应用备注
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Bioimaging
1. Seed the cells in appropriate culture medium at ~10,000 cells per well in an 96-well Imaging Plate and culture overnight.
2. Remove the culture medium from the wells, and fix the cells by adding 100 myl of Fixation Buffer to each well. Incubate for 10 minutes at room temperature (RT).
3. Remove the fixative from the wells, and permeabilize the cells using either 90% methanol, or Triton™ X-100: a. Add 100 myl of -20°C 90% methanol to each well and incubate for 5 minutes at RT. OR b. Add 100 myl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT.
4. Remove the permeabilization buffer, and wash the wells twice with 100 myl of 1× PBS.
5. Remove the PBS, and block the cells by adding 100 myl of to each well. Incubate for 30 minutes at RT.
6. Remove the blocking buffer and add 50 myl of the optimally titrated primary antibody (diluted in Stain Buffer) to each well, and incubate for 1 hour at RT.
7. Remove the primary antibody, and wash the wells three times with 100 myl of 1× PBS.
8. Remove the PBS, and add the second step reagent at its optimally titrated concentration in 50 myl to each well, and incubate in the dark for 1 hour at RT.
9. Remove the second step reagent, and wash the wells three times with 100 myl of 1× PBS.
10. Remove the PBS, and counter-stain the nuclei by adding 200 myl per well of 2 myg/ml Hoechst 33342 in 1× PBS to each well at least 15 minutes before imaging.
11. View and analyze the cells on an appropriate imaging instrument. - 说明
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Related Products: ABIN967389, ABIN968535
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 250 μg/mL
- 缓冲液
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store undiluted at -20°C.
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: "Human phosphoinositide 3-kinase C2beta, the role of calcium and the C2 domain in enzyme activity." in: The Journal of biological chemistry, Vol. 273, Issue 49, pp. 33082-90, (1999) (PubMed).
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: "Human phosphoinositide 3-kinase C2beta, the role of calcium and the C2 domain in enzyme activity." in: The Journal of biological chemistry, Vol. 273, Issue 49, pp. 33082-90, (1999) (PubMed).
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- 抗原
- PIK3C2B (Phosphatidylinositol-4-Phosphate 3-Kinase, Catalytic Subunit Type 2 beta (PIK3C2B))
- 别名
- PI3-Kinase C2 beta (PIK3C2B 产品)
- 别名
- C2-PI3K antibody, phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 beta antibody, phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 beta antibody, Pik3c2b antibody, PIK3C2B antibody
- 背景
- Phosphatidylinositol (PtdIns) (3) kinase phosphorylates the D-3 position of the inositolring of PtdIns, producing PtdIns(3)P, PtdIns(3,4)P2, and PtdIns(3,4,5)P3. PI3-kinase is a heterodimer of an 85 kDa regulatory subunit (p85) and a 110 kDa catalytic subunit (p110). However, it is only one member of a larger family of proteins with similarity to the p110 subunit. These different PI3-kinase isoforms have been divided into three classes. Class I consists of p110alpha and p110ß which bind the p85 subunit and associate with receptor tyrosine kinases. Class II includes 68D and cpk from Drosophila, p170 and cpk-m from mouse, and C2alpha, C2ß (HsC2), and C2gamma from human. These proteins phosphorylate PtdIns and PtdIns(4)P, but not PtdIns(4,5)P2, and each contain a C-terminal C2 domain that may negatively regulate the catalytic domain. Class III members only phosphorylate PtdIns to PtdIns(3)P and include the S. cerevisiae Vps34p and its human homologs. In humans, the class II PI3-kinases C2alpha and C2ß have similar catalytic, PI kinase, and C2 domains. However they differ in their N-terminal regions. In addition, C2ß has no cation specificity, while C2alpha prefers Mg2+-ATP for optimal phosphorylation.
- 分子量
- 165 kDa
- 途径
- Inositol Metabolic Process
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