EPS15 抗体 (AA 694-888)
(5 references)-
- 抗原 See all EPS15 抗体
- EPS15 (Epidermal Growth Factor Receptor Pathway Substrate 15 (EPS15))
-
抗原表位
- AA 694-888
-
适用
- 人, 小鼠, 大鼠
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This EPS15 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)
- 交叉反应
- 小鼠, 大鼠
- 产品特性
-
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Human Eps15 aa. 694-888
- 克隆位点
- 17-eps15
- 亚型
- IgG2a
- Top Product
- Discover our top product EPS15 Primary Antibody
-
anti-Epidermal Growth Factor Receptor Pathway Substrate 15 (EPS15) antibody
EPS15 适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor Pathway Substrate 15 (EPS15) (pTyr849) antibodyEPS15 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor Pathway Substrate 15 (EPS15) (pTyr849) antibodyEPS15 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor Pathway Substrate 15 (EPS15) (pTyr849) antibodyEPS15 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor Pathway Substrate 15 (EPS15) (AA 794-843) antibodyEPS15 适用: 人, 小鼠, 大鼠, Pig, Cow, 马, 猴, 兔, 豚鼠, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor Pathway Substrate 15 (EPS15) (AA 2-88) antibodyEPS15 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Epidermal Growth Factor Receptor Pathway Substrate 15 (EPS15) (C-Term) antibodyEPS15 适用: 人, 小鼠, 大鼠, Pig, Cow, 马, 犬, 兔, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated
-
- 说明
-
Related Products: ABIN968537, ABIN967389
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 250 μg/mL
- 缓冲液
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store undiluted at -20° C.
-
-
: "The actin-binding protein Hip1R associates with clathrin during early stages of endocytosis and promotes clathrin assembly in vitro." in: The Journal of cell biology, Vol. 154, Issue 6, pp. 1209-23, (2001) (PubMed).
: "Regulation of complex formation of POB1/epsin/adaptor protein complex 2 by mitotic phosphorylation." in: The Journal of biological chemistry, Vol. 275, Issue 24, pp. 18399-406, (2000) (PubMed).
: "Crystal structure of the alpha appendage of AP-2 reveals a recruitment platform for clathrin-coat assembly." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 16, pp. 8907-12, (1999) (PubMed).
: "Mapping of the molecular determinants involved in the interaction between eps15 and AP-2." in: Cancer research, Vol. 57, Issue 2, pp. 240-5, (1997) (PubMed).
: "Structural requirements of the epidermal growth factor receptor for tyrosine phosphorylation of eps8 and eps15, substrates lacking Src SH2 homology domains." in: The Journal of biological chemistry, Vol. 270, Issue 27, pp. 16271-6, (1995) (PubMed).
-
: "The actin-binding protein Hip1R associates with clathrin during early stages of endocytosis and promotes clathrin assembly in vitro." in: The Journal of cell biology, Vol. 154, Issue 6, pp. 1209-23, (2001) (PubMed).
-
- 抗原
- EPS15 (Epidermal Growth Factor Receptor Pathway Substrate 15 (EPS15))
- 别名
- Eps15 (EPS15 产品)
- 别名
- AF-1P antibody, AF1P antibody, MLLT5 antibody, CG16932 antibody, Dmel\\CG16932 antibody, EH1 antibody, EPS-15 antibody, Eps15 antibody, anon-WO0118547.85 antibody, eps-15 antibody, eps15 antibody, i31 antibody, 2410112D09Rik antibody, RGD1307641 antibody, epidermal growth factor receptor pathway substrate 15 antibody, Epidermal growth factor receptor pathway substrate clone 15 antibody, hypothetical protein antibody, EPS15 antibody, Eps-15 antibody, eps15 antibody, Eps15 antibody, PGTG_06574 antibody
- 背景
- The eps15 (EGF receptor pathway substrate clone number 15) gene was isolated using an expression cloning method designed for direct isolation of cDNAs encoding substrates of tyrosine kinases. The eps15 gene encodes a 145 kDa protein that is tyrosine phosphorylated following EGF receptor activation and this receptor directly phosphorylates purified Eps15 in vitro. Phosphorylation of Eps15 is much more efficient following EGF receptor activation versus erbB2 kinase activation, suggesting the protein is predominantly a part of an EGF receptor signaling pathway. Overexpression of Eps15 causes transformation of NIH 3T3 cells, implying Eps15 may be involved in the control of cell proliferation. Eps15 binds to the SH3 domain of Crk through its proline-rich domain at the C-terminal region. Adaptin alpha binds to the C-terminus of Eps15 at three different sites ranging from residues 650 to 730. Eps15, Adaptin alpha, and EGF receptor are co-localized at the clathrin-coated pits after receptor activation. Therefore, Eps15 may recruit EGF receptors to the clathrin-coated pits for internalization of the activated receptor. This antibody is routinely tested by western blot analysis.
- 分子量
- 145 kDa
- 途径
- EGFR Signaling Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, EGFR Downregulation
-
