(11 references)-
- 抗原 See all L-Selectin (SELL) 抗体
- L-Selectin (SELL) (Selectin L (SELL))
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适用
- 化学剂
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This L-Selectin antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Western Blotting (WB), Functional Studies (Func)
- 品牌
- BD Pharmingen™
- 产品特性
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1. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3. Please refer to us for technical protocols. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Peripheral Blood T Lymphocytes
- 克隆位点
- SK11
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product SELL Primary Antibody
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anti-Selectin L (SELL) (AA 301-372) antibody
SELL 适用: 小鼠, 大鼠 FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Selectin L (SELL) (AA 1-100) antibodySELL 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Selectin L (SELL) antibody (APC)SELL 适用: 人 FACS 宿主: 小鼠 Monoclonal LT-TD180 APC
anti-Selectin L (SELL) (AA 83-186) antibodySELL 适用: 人 FACS, WB, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 8C8B7 unconjugated
anti-Selectin L (SELL) (AA 109-346) antibodySELL 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Selectin L (SELL) antibodySELL 适用: 人, Cow FACS, IP, ICC 宿主: 小鼠 Monoclonal IVA94 unconjugated
anti-Selectin L (SELL) antibody (FITC)SELL 适用: 人 FACS 宿主: 小鼠 Monoclonal DREG56 FITC
anti-Selectin L (SELL) antibodySELL 适用: 小鼠 FACS, IP, IHC (fro), ICC, IHC (p) 宿主: 大鼠 Monoclonal Mel-14 unconjugated
anti-Selectin L (SELL) antibody (FITC)SELL 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal Mel-14 FITC
anti-Selectin L (SELL) antibodySELL 适用: 小鼠 FACS, IP, IHC (fro), Func, ICC, IHC (p) 宿主: 大鼠 Monoclonal Mel-14 unconjugated
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- 应用备注
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Functional Studies:
Anti-LECAM-1 monoclonal antibodies have been shown to inhibit neutrophil localization at sites of acute inflammation. Anti-LECAM-1 monoclonal antibody (clone SK11) also induces suppressor function in CD4+ T lymphocytes but does not induce lymphocyte proliferation.
Immunoblotting:
Using anti-LECAM-1 monoclonal antibody (clone SK11) in Western blot analysis of extracts from Leu- 8-transfected COS cells and the human T-cell leukemia line (Jurkat) revealed at least seven species of similar molecular weight and at least two closely spaced bands of 76 kD, the largest molecular weight form seen in COS cells. - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4° C.
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: "Selectins: a family of adhesion receptors." in: Cell, Vol. 67, Issue 2, pp. 233, (1991) (PubMed).
: "Leukocytes roll on a selectin at physiologic flow rates: distinction from and prerequisite for adhesion through integrins." in: Cell, Vol. 65, Issue 5, pp. 859-73, (1991) (PubMed).
: "The neutrophil selectin LECAM-1 presents carbohydrate ligands to the vascular selectins ELAM-1 and GMP-140." in: Cell, Vol. 66, Issue 5, pp. 921-33, (1991) (PubMed).
: "CD62 and endothelial cell-leukocyte adhesion molecule 1 (ELAM-1) recognize the same carbohydrate ligand, sialyl-Lewis x." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 14, pp. 6224-8, (1991) (PubMed).
: "Chemotactic factors regulate lectin adhesion molecule 1 (LECAM-1)-dependent neutrophil adhesion to cytokine-stimulated endothelial cells in vitro." in: The Journal of clinical investigation, Vol. 87, Issue 2, pp. 609-18, (1991) (PubMed).
: "The peripheral lymph node homing receptor, LECAM-1, is involved in CD18-independent adhesion of human neutrophils to endothelium." in: Biochemical and biophysical research communications, Vol. 174, Issue 1, pp. 236-43, (1991) (PubMed).
: "Function and regulation of the neutrophil MEL-14 antigen in vivo: comparison with LFA-1 and MAC-1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 143, Issue 10, pp. 3318-24, (1989) (PubMed).
: "Leu-8/TQ1 is the human equivalent of the Mel-14 lymph node homing receptor." in: Nature, Vol. 342, Issue 6245, pp. 78-82, (1989) (PubMed).
: "Induction of CD4 suppressor T cells with anti-Leu-8 antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 139, Issue 1, pp. 49-54, (1987) (PubMed).
: "Leukocyte-endothelial cell recognition: evidence of a common molecular mechanism shared by neutrophils, lymphocytes, and other leukocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 12, pp. 4313-21, (1987) (PubMed).
: "Expression of the Leu-8 antigen by B-cell lymphomas." in: American journal of clinical pathology, Vol. 88, Issue 4, pp. 486-90, (1987) (PubMed).
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: "Selectins: a family of adhesion receptors." in: Cell, Vol. 67, Issue 2, pp. 233, (1991) (PubMed).
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- 抗原
- L-Selectin (SELL) (Selectin L (SELL))
- 别名
- CD62L (L-selectin) (SELL 产品)
- 别名
- CD62L antibody, LAM1 antibody, LECAM1 antibody, LEU8 antibody, LNHR antibody, LSEL antibody, LYAM1 antibody, PLNHR antibody, TQ1 antibody, A.11 antibody, L-selectin antibody, LECAM-1 antibody, SELE antibody, AI528707 antibody, Lnhr antibody, Ly-22 antibody, Ly-m22 antibody, Lyam-1 antibody, Lyam1 antibody, selectin L antibody, selectin, lymphocyte antibody, SELL antibody, Sell antibody
- 物质类
- Chemical
- 背景
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Anti-LECAM-1 (Leu-8/TQ1) monoclonal antibody (clone SK11) recognizes an 80 kD protein on the surface of human leukocytes. The leukocyte-endothelial cell adhesion molecule 1 (LECAM-1) protein is the human homologue of the murine lymph node homing receptor, MEL-14, a molecule that initiates the adhesion of circulating leukocytes to high endothelium in postcapillary venules. LECAM-1 belongs to the selectin family of cell adhesion molecules and is also known as CD62L. This antibody is routinely tested by flow cytometric analysis.
Synonyms: L-Selectin
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