Vbeta 8 TCR 抗体

ABIN967386 产品详细信息, 供应商: Log in to see
抗原
适用
小鼠
1
宿主
小鼠
1
克隆类型 (克隆位点)
单克隆 ()
标记
非结合性
1
应用范围
Blocking Reagent (BR), Flow Cytometry (FACS), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
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品牌 BD Pharmingen™
免疫原 BALB.B Mouse Lymph-Node and Spleen T Cells
克隆位点 F23-1
亚型 IgG2a, kappa
产品特性 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE™ (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
纯化方法 The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
背景 The F23.1 antibody reacts with the Vbeta 8.1, Vbeta 8.2, and Vbeta 8.3 T-cell receptors (TCR) of mice having the b haplotype (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C58, DBA/1, DBA/2) of the Tcrb gene complex. The Tcrb-V8 subfamily gene loci are deleted in mice having the a (e.g., C57BR, C57L, SJL, SWR) or c (e.g., RIII) haplotype. Vbeta 8.1 TCR-bearing T lymphocytes are clonally eliminated in mice expressing superantigen coded by Mtv-7 (Mls-1a, Mlsa) provirus (e.g., AKR, CBA/J, C58, DBA/2), and activation or elimination of Vbeta 8.1 TCR-expressing T cells by this determinant is partially dependent upon presentation by I-E. Mtv-43 and/or exogenous MMTV-SW superantigens also cause incomplete elimination of Vbeta 8.1 TCR-bearing T cells. In addition to expression on conventional T lymphocytes, Vbeta 8.2 is the predominant beta chain of the TCR on NK-T cells. Staphylococcal enterotoxin B, in association with antigen-presenting cells expressing I-A and/or I-E, stimulates lymphocytes bearing Vbeta 8 TCR and selectively eliminates those T cells in vivo. Soluble and plate-bound F23.1 antibody activates Vbeta 8 TCR-bearing T cells, soluble antibody blocks cytolysis mediated by Vbeta 8 TCR-bearing cytotoxic T lymphocytes, and in vivo treatment of neonatal mice can arrest intrathymic maturation of Vbeta 8 TCR-bearing T cells. This antibody is routinely tested by flow cytometric analysis.
应用备注 For flow cytometry of cell suspensions from peripheral lymphoid tissues, it is recommended that multicolor staining be performed to distinguish T lymphocytes from non-T cells.
限制 仅限研究用
状态 Liquid
浓度 0.5 mg/mL
缓冲液 Aqueous buffered solution containing ≤0.09 % sodium azide.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C
储存方法 Store undiluted at 4° C.
厂商提供的图像
Flow Cytometry (FACS) image for anti-Vbeta 8 TCR 抗体 (ABIN967386) Two-color analysis of the expression of Vbeta 8 TCR on peripheral lymphocytes. C57BL/...
Flow Cytometry (FACS) image for anti-Vbeta 8 TCR 抗体 (ABIN967386) Two-color analysis of the expression of Vbeta 8 TCR on peripheral lymphocytes. C57BL/...
有引用在: Hodes, Abe: "Mouse endogenous superantigens: Ms and Mls-like determinants encoded by mouse retroviruses." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Appendix 1, pp. Appendix 1F, 2008 (PubMed).

Jiang, Möller: "In vitro effects of HgCl2 on murine lymphocytes. II. Selective activation of T cells expressing certain V beta TCR." in: International immunology, Vol. 8, Issue 11, pp. 1729-36, 1997 (PubMed).

Brodnicki, Holman, Kranz: "Reactivity and epitope mapping of single-chain T cell receptors with monoclonal antibodies." in: Molecular immunology, Vol. 33, Issue 3, pp. 253-63, 1996 (PubMed).

Mogil, Radvanyi, Gonzalez-Quintial, Miller, Mills, Theofilopoulos, Green: "Fas (CD95) participates in peripheral T cell deletion and associated apoptosis in vivo." in: International immunology, Vol. 7, Issue 9, pp. 1451-8, 1996 (PubMed).

Renno, Hahne, Tschopp, MacDonald: "Peripheral T cells undergoing superantigen-induced apoptosis in vivo express B220 and upregulate Fas and Fas ligand." in: The Journal of experimental medicine, Vol. 183, Issue 2, pp. 431-7, 1996 (PubMed).

Bendelac: "Mouse NK1+ T cells." in: Current opinion in immunology, Vol. 7, Issue 3, pp. 367-74, 1995 (PubMed).

Hugo, Kappler, Godfrey, Marrack: "Thymic epithelial cell lines that mediate positive selection can also induce thymocyte clonal deletion." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 3, pp. 1022-31, 1994 (PubMed).

Saint-Ruf, Ungewiss, Groettrup, Bruno, Fehling, von Boehmer: "Analysis and expression of a cloned pre-T cell receptor gene." in: Science (New York, N.Y.), Vol. 266, Issue 5188, pp. 1208-12, 1994 (PubMed).

Yagi, Nakata, Uchiyama, Nishikawa, Mizushima, Nishioka, Ito, Yagita, Okumura, Janeway: "Superantigen-like properties of an antibody bispecific for MHC class II molecules and the V beta domain of the T cell antigen receptor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 8, pp. 3833-41, 1994 (PubMed).

Wolff, Hong, von Grafenstein, Janeway: "TCR-CD4 and TCR-TCR interactions as distinctive mechanisms for the induction of increased intracellular calcium in T-cell signalling." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 3, pp. 1337-45, 1993 (PubMed).

Haqqi, Banerjee, Anderson, David: "RIII S/J (H-2r). An inbred mouse strain with a massive deletion of T cell receptor V beta genes." in: The Journal of experimental medicine, Vol. 169, Issue 6, pp. 1903-9, 1989 (PubMed).

Kyewski, Schirrmacher, Allison: "Antibodies against the T cell receptor/CD3 complex interfere with distinct intra-thymic cell-cell interactions in vivo: correlation with arrest of T cell differentiation." in: European journal of immunology, Vol. 19, Issue 5, pp. 857-63, 1989 (PubMed).

White, Herman, Pullen, Kubo, Kappler, Marrack: "The V beta-specific superantigen staphylococcal enterotoxin B: stimulation of mature T cells and clonal deletion in neonatal mice." in: Cell, Vol. 56, Issue 1, pp. 27-35, 1989 (PubMed).

Kappler, Staerz, White, Marrack: "Self-tolerance eliminates T cells specific for Mls-modified products of the major histocompatibility complex." in: Nature, Vol. 332, Issue 6159, pp. 35-40, 1988 (PubMed).

Behlke, Henkel, Anderson, Lan, Hood, Braciale, Braciale, Loh: "Expression of a murine polyclonal T cell receptor marker correlates with the use of specific members of the V beta 8 gene segment subfamily." in: The Journal of experimental medicine, Vol. 165, Issue 1, pp. 257-62, 1987 (PubMed).

Behlke, Chou, Huppi, Loh: "Murine T-cell receptor mutants with deletions of beta-chain variable region genes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 3, pp. 767-71, 1986 (PubMed).

Staerz, Rammensee, Benedetto, Bevan: "Characterization of a murine monoclonal antibody specific for an allotypic determinant on T cell antigen receptor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 134, Issue 6, pp. 3994-4000, 1985 (PubMed).