Specific for the ~32k DARPP-32 protein phosphorylated at Ser137. Immunolabeling is blocked by by preadsorption with the phospho-peptide used as antigen but not by the dephospho-peptide.
交叉反应 (详细)
Species reactivity (expected):Bovine, Canine, Chicken, Human, Mouse and non-Human Primates. Species reactivity (tested):Rat.
纯化方法
Affinity Chromatography.
免疫原
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser137 of Rat DARPP-32.
DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac® (Maldve et al., 2002, Lindskog et al., 2002, Svenningsson et al., 2002). Serine 137 is phosphorylated by casein kinase 1 and phosphorylation of this site is increased following acute administration of Prozac® (Svenningsson et al., 2002).Synonyms: DARPP-32, Dopamine- and cAMP-regulated neuronal phosphoprotein, FLJ20940, PPP1R1B, Protein phosphatase 1 regulatory subunit 1B