Amylase, alpha 抗体 (HRP)
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- 抗原 See all Amylase, alpha (AMY) 抗体
- Amylase, alpha (AMY)
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适用
- Bacillus amyloliquefaciens
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Amylase, alpha antibody is conjugated to HRP
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应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- 交叉反应 （详细）
- Cross reactivity against a-Amylase from other tissues and species is unknown.
- 纯化方法
- Alpha Amylase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified a-Amylase [Bacillus amyloliquefaciens].
- 免疫原
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Immunogen: Anti-Alpha Amylase Antibody was produced by repeated immunizations with Bacillus amyloliquefaciens a-Amylase protein.
Immunogen Type: Native Protein
- 亚型
- IgG
- Top Product
- Discover our top product AMY Primary Antibody
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anti-Amylase, alpha (AMY) antibody (Biotin)
AMY 适用: Bacillus amyloliquefaciens WB, ELISA, IP 宿主: 兔 Polyclonal Biotin
anti-Amylase, alpha (AMY) antibodyAMY 适用: Oryza sativa WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Application Note: Alpha Amylase antibody has been assayed against 1.0 μg of Alpha Amylase [Bacillus amyloliquefaciens] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Amylase, alpha (AMY)
- 别名
- Alpha Amylase (AMY 产品)
- 别名
- LOC663954 antibody, LOC100136478 antibody, alpha amylase antibody, alpha-amylase antibody, LOC663954 antibody, HQ_RS05305 antibody, LOC100136478 antibody
- 背景
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Synonyms: Alpha-amylase, EC=3.2.1.1, 1,4-alpha-D-glucan glucanohydrolase
Background: Anti-Alpha Amylase Antibody detects Alpha Amylase. α-Amylase is an enzyme that hydrolyses alpha-bonds of large alpha-linked polysaccharides, such as starch and glycogen, yielding glucose and maltose. Anti-Alpha Amylase Antibody is ideal for investigators involved in Cell Signaling.
Gene Name: Alpha-amylase
- 基因ID
- 7849308
- UniProt
- P00692
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