GATA3 抗体 (N-Term)
-
- 抗原 See all GATA3 抗体
- GATA3 (GATA Binding Protein 3 (GATA3))
-
抗原表位
- N-Term
-
适用
- 人, 小鼠, 大鼠, Cow, Pig, 犬
-
宿主
- 山羊
-
克隆类型
- 多克隆
-
标记
- This GATA3 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- 序列
- EVTADQPRWV SHH-C
- 特异性
- This antibody detects GATA3 at N-term. It is expected to recognize both reported isoforms (NP_001002295.1 and NP_002042.1).
- 交叉反应 (详细)
-
Species reactivity (expected):Mouse, Rat, Dog, Pig, Cow.
Species reactivity (tested):Human. - 纯化方法
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
- 免疫原
- Peptide from the N Terminus of the protein sequence according to NP_001002295.1, NP_002042.1 . Genename: GATA3
- Top Product
- Discover our top product GATA3 Primary Antibody
-
-
- 应用备注
-
Peptide ELISA: Antibody detection limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 50 kDa band observed in lysates of cell line MOLT4(calculated MW of 48.0 according to NP_001002295.1 and 47.0 kDa according toNP_002042.1). Immunohistochemistry: In paraffin embedded Human Tonsil, stains nuclei of some cells inthe interfollicular area and in germinal centres. Recommended concentration, 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
-
- 浓度
- 0.5 mg/mL
- 缓冲液
- Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
-
-
Mycoleptodonoides aitchisonii suppresses asthma via Th2 and Th1 cell regulation in an ovalbumin‑induced asthma mouse model." in: Molecular medicine reports, Vol. 17, Issue 1, pp. 11-20, (2018) (PubMed).
: "Erythronium japonicum attenuates histopathological lung abnormalities in a mouse model of ovalbumin-induced asthma." in: International journal of molecular medicine, Vol. 37, Issue 5, pp. 1221-8, (2016) (PubMed).
: "Bacillus subtilis KCTC 11782BP-Produced Alginate Oligosaccharide Effectively Suppresses Asthma via T-Helper Cell Type 2-Related Cytokines." in: PLoS ONE, Vol. 10, Issue 2, pp. e0117524, (2015) (PubMed).
: "
-
Mycoleptodonoides aitchisonii suppresses asthma via Th2 and Th1 cell regulation in an ovalbumin‑induced asthma mouse model." in: Molecular medicine reports, Vol. 17, Issue 1, pp. 11-20, (2018) (PubMed).
-
- 抗原
- GATA3 (GATA Binding Protein 3 (GATA3))
- 别名
- GATA3 (GATA3 产品)
- 别名
- HDR antibody, HDRS antibody, Gata-3 antibody, jal antibody, cb550 antibody, gta3 antibody, GATA-3 antibody, XGATA3 antibody, xgata-3 antibody, hdr antibody, xGATA-3 antibody, GATA3 antibody, GATA transcription factor 3 antibody, T4L20.260 antibody, T4L20_260 antibody, gata3 antibody, ZH-DR antibody, zgc:103531 antibody, GATA binding protein 3 antibody, GATA binding protein 3 S homeolog antibody, GATA transcription factor 3 antibody, amidase antibody, putative amidase antibody, GATA-binding protein 3 antibody, hematopoietic death receptor antibody, GATA3 antibody, Gata3 antibody, gata3 antibody, gata3.S antibody, gatA3 antibody, hdr antibody
- 背景
- The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.Synonyms: GATA-3, GATA-binding factor 3, Trans-acting T-cell-specific transcription factor GATA-3
- 基因ID
- 2625
- NCBI登录号
- NP_001002295
- UniProt
- P23771
- 途径
- Hormone Transport, Regulation of Hormone Metabolic Process, Tube Formation
-