This antibody detects endogenous levels of total PI3K P85 only when phosphorylated at Tyrosine 467.
纯化方法
Affinity Chromatography using epitope-specific phosphopeptide.
免疫原
The antiserum was produced against synthesized phosphopeptide derived from human PI3K P85 around the phosphorylation site of Tyrosine 467 (L-YP-E-E-Y).
Suitable for use in in Western blot (1/500-1/1000). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
缓冲液
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 alpha). p85 alpha is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 alpha is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.Synonyms: PI3-kinase subunit p85-alpha, PI3K regulatory subunit alpha, Phosphatidylinositol 3-kinase regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha