Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
特异性
The antibody detects endogenous level of LIMK2 only when phosphorylated at threonine 505.
纯化方法
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
免疫原
Peptide sequence around phosphorylation site of pThr505 (R-Y-T (p) -V-V) derived from Human LIMK2. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.