This Tyrosine Hydroxylase antibody is un-conjugated
应用范围
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
特异性
Specific for the ~60k tyrosine hydroxylase protein phosphorylated at Ser40. Some higher molecular weight bands may be detected by the antibody depending upon the brain region being studied, protein loads and the detection methods used. The antibody has three orders of magnitude selectiity over dephospho TH.
交叉反应
哺乳动物
纯化方法
Antigen Affinity Purified from Pooled Serum
免疫原
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser40 conjugated to KLH
Recommended Dilution: WB: 1:1000 IF (frozen sections, Witkovsky et al., 2000): 1:1000 IHC (frozen sections, Witkovsky et al., 2000): 1:1000 Quality Control: Western blots performed on each lot.
限制
仅限研究用
状态
Liquid
缓冲液
100 μL in 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.
储存条件
-20 °C
Salvatore, Calipari, Jones: "Regulation of Tyrosine Hydroxylase Expression and Phosphorylation in Dopamine Transporter-Deficient Mice." in: ACS chemical neuroscience, Vol. 7, Issue 7, pp. 941-51, (2017) (PubMed).
Salvatore, Terrebonne, Fields, Nodurft, Runfalo, Latimer, Ingram: "Initiation of calorie restriction in middle-aged male rats attenuates aging-related motoric decline and bradykinesia without increased striatal dopamine." in: Neurobiology of aging, Vol. 37, pp. 192-207, (2016) (PubMed).
Calipari, Ferris, Melchior, Bermejo, Salahpour, Roberts, Jones: "Methylphenidate and cocaine self-administration produce distinct dopamine terminal alterations." in: Addiction biology, Vol. 19, Issue 2, pp. 145-55, (2014) (PubMed).
Izumi, Ezumi, Takada-Takatori, Akaike, Kume: "Endogenous dopamine is involved in the herbicide paraquat-induced dopaminergic cell death." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 139, Issue 2, pp. 466-78, (2014) (PubMed).
Salvatore, Pruett: "Dichotomy of Tyrosine Hydroxylase and Dopamine Regulation between Somatodendritic and Terminal Field Areas of Nigrostriatal and Mesoaccumbens Pathways." in: PLoS ONE, Vol. 7, Issue 1, pp. e29867, (2012) (PubMed).
McCutcheon, Conrad, Carr, Ford, McGehee, Marinelli: "Dopamine neurons in the ventral tegmental area fire faster in adolescent rats than in adults." in: Journal of neurophysiology, Vol. 108, Issue 6, pp. 1620-30, (2012) (PubMed).
Salvatore, Pruett, Dempsey, Fields: "Comprehensive profiling of dopamine regulation in substantia nigra and ventral tegmental area." in: Journal of visualized experiments : JoVE, Issue 66, (2012) (PubMed).
Perez-Costas, Melendez-Ferro, Rice, Conley, Roberts: "Dopamine pathology in schizophrenia: analysis of total and phosphorylated tyrosine hydroxylase in the substantia nigra." in: Frontiers in psychiatry, Vol. 3, pp. 31, (2012) (PubMed).
Nakashima, Mori, Kaneko, Hayashi, Nagatsu, Ota: "Phosphorylation of the N-terminal portion of tyrosine hydroxylase triggers proteasomal digestion of the enzyme." in: Biochemical and biophysical research communications, Vol. 407, Issue 2, pp. 343-7, (2011) (PubMed).
Meyer, Richer, Benkovic, Hayashi, Kansy, Hale, Moy, Kim, OCallaghan, Tsai, Greengard, Nairn, Cowan, Miller, Antich, Bibb: "Striatal dysregulation of Cdk5 alters locomotor responses to cocaine, motor learning, and dendritic morphology." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 47, pp. 18561-6, (2008) (PubMed).
Jedynak, Ali, Haycock, Hope: "Acute administration of cocaine regulates the phosphorylation of serine-19, -31 and -40 in tyrosine hydroxylase." in: Journal of neurochemistry, Vol. 82, Issue 2, pp. 382-8, (2002) (PubMed).
Salvatore, Waymire, Haycock: "Depolarization-stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ." in: Journal of neurochemistry, Vol. 79, Issue 2, pp. 349-60, (2001) (PubMed).
Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines Dopamine and Norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson?s disease and drug abuse (Kish et al., 2001, Zhu et al., 2000, Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000, Salvatore et al., 2001, Dunkley et al., 2004). The activity of TH is also regulated by phosphorylation (Haycock et al., 1982, Haycock et al., 1992, Jedynak et al., 2002). Phospho-specific antibodies for the phosphorylation sites on TH can be used to great effect in studying this regulation and in identifying the cells in which TH phosphorylation occurs. Anti-Phospho Ser40 Tyrosine Hydroxylase Western blot of recombinant phospho- and dephospho-TH showing selective immunolabeling by the phospho-specific antibody of the ~60k TH phosphorylated at Ser40. The pan-specific antibody (anti-pan-TH) recognized both the phospho- and dephospho-TH, while most importantly, the phospho-specific antibody (anti-Ser40 TH) recognized only phospho-TH. Immunohistochemical staining of retina with the pan-tyrosine hydroxylase (pan-TH) and phospho-specific tyrosine hydroxylase (phospho-TH) antibodies. The pan-TH antibody shows extensive labeling in this photomicrograph of the retina. In contrast, the phospho-TH antibody selectively labels only the two amacrine cells in this light-stimulated retina example.