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- 抗原 See all CREB1 抗体
- CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))
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抗原表位
- pSer133
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适用
- 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CREB1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC)
- 特异性
- Specific for the ~45k CREB protein phosphorylated at Ser133. Immunolabeling is blocked by (-phosphatase treatment.
- 交叉反应
- 大鼠
- 预测反应
- bovine, canine, chicken, human, mouse, non-human primate, rat, sheep, Xenopus, zebra fish
- 纯化方法
- Antigen Affinity Purified from Pooled Serum
- 免疫原
- Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser133 conjugated to KLH
- Top Product
- Discover our top product CREB1 Primary Antibody
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- 应用备注
- Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 μL in 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.
- 储存条件
- -20 °C
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Preserving protein profiles in tissue samples: differing outcomes with and without heat stabilization." in: Journal of neuroscience methods, Vol. 196, Issue 1, pp. 99-106, (2011) (PubMed).
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Preserving protein profiles in tissue samples: differing outcomes with and without heat stabilization." in: Journal of neuroscience methods, Vol. 196, Issue 1, pp. 99-106, (2011) (PubMed).
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- 抗原
- CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))
- 别名
- CREB1 (CREB1 产品)
- 别名
- creb antibody, CREB1 antibody, Creb1 antibody, CREB antibody, 2310001E10Rik antibody, 3526402H21Rik antibody, AV083133 antibody, Creb antibody, Creb-1 antibody, creb1 antibody, zgc:55598 antibody, cAMP responsive element binding protein 1 antibody, cAMP responsive element binding protein antibody, cAMP responsive element binding protein 1 S homeolog antibody, cAMP responsive element binding protein 1a antibody, CREB1 antibody, creb1 antibody, CREB antibody, Creb1 antibody, creb1.S antibody, creb1a antibody
- 背景
- It is well known that the control of gene expression involves activation of protein kinase cascades that regulate transcription factors within the nucleus (Karin and Hunter, 1995). The cyclic AMP response element binding protein (CREB) is one of the best characterized stimulus-induced transcription factors (Montminy, 1997). This transcription factor is a component of intracellular signaling events that regulate a wide range of biological functions, from spermatogenesis to circadian rhythms and memory (Shaywitz and Greenberg, 1999, Silva et al., 1998). A variety of protein kinases including protein kinase A (PKA), mitogen-activated protein kinases (MAPKs), and Ca2+/calmodulin-dependent protein kinases (CaMKs) phosphorylate CREB at serine 133 (Ser133), and phosphorylation of Ser133 are required for CREB-mediated transcription (Johannessen et al., 2004, Kornhauser et al., 2002). Anti-Phospho-Ser133 CREB Western blot of a forskolin stimulated rat hippocampal lysate showing specific immunolabeling of the ~45k CREB phosphorylated at Ser133 (Left Lane). The right lane is indicative that the specific immunolabeling of the CREB protein is completely eliminated by treatment with lambda phosphatase ((-Ptase, 1200 units for 30 min).
- 分子量
- '45 kDa
- 基因ID
- 81646
- UniProt
- P15337
- 途径
- TLR signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation
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