V5 Epitope 标记 抗体 (HRP)

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抗原
适用
标记
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宿主
小鼠
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克隆类型 (克隆位点)
单克隆 ()
标记
HRP
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应用范围
Enzyme Immunoassay (EIA), Western Blotting (WB)
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免疫原 Mice were infected with the paramyxovirus SV5, Simian-Virus 5. Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 Ag14 myeloma cell line.
序列 GKPIPNPLLG LDST
克隆位点 SV5-Pk1
亚型 IgG2a
纯化方法 Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
别名 Pk (V5) Epitope Tag (GKPIPNPLLGLDST)
物质类 Tag
研究领域 Tags/Labels
应用备注 ELISA. Western Blot (1/1000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
浓度 1.0 mg/mL
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
背景 Hanke, Szawlowski, Randall: "Construction of solid matrix-antibody-antigen complexes containing simian immunodeficiency virus p27 using tag-specific monoclonal antibody and tag-linked antigen." in: The Journal of general virology, Vol. 73 ( Pt 3), pp. 653-60, 1992 (PubMed).

Southern, Young, Heaney, Baumgärtner, Randall: "Identification of an epitope on the P and V proteins of simian virus 5 that distinguishes between two isolates with different biological characteristics." in: The Journal of general virology, Vol. 72 ( Pt 7), pp. 1551-7, 1991 (PubMed).

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