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ATP6V0D2 抗体 (Isoform 1)

Name: ATP6V0D2 抗体 (Isoform 1)
SKU: ABIN3197488
Availability: OutOfStock

ATP6V0D2 适用: Tomato, Arabidopsis thaliana, Zea mays, Pteris vittata (Chinese ladder brake), Oryza sativa, Lilium longiflorum (Trumpet lily), Chlamydomonas reinhardtii, Barley WB, IHC 宿主: 兔 Polyclonal unconjugated

Western Blotting: 1 - molecular weight markers (Precision Plus Protein Standards, Dual Color, Bio-Rad, 10ul), 2 -Arabidopsis thaliana leaf extract (25 ug) Experimental Conditions: Protein was separated on a 12% SDS-PAGE gel at 200 V for approximately 40 min using the mini-Protein 3 cell (Bio-Rad).

Western Blot analysis of samples from yeast whole cell extract expressing the Arabidopsis thaliana subunit E1.

2 images

(3 references)

产品编号 ABIN3197488

发货至: 中国

Datasheet as PDF

抗原 See all ATP6V0D2 抗体

ATP6V0D2 (ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2))
抗原表位
15

2

2

1

1

1

1
Isoform 1
适用
23

5

5

4

4

4

3

3

2

1

1

1

1

1

1
Tomato, Arabidopsis thaliana, Zea mays, Pteris vittata (Chinese ladder brake), Oryza sativa, Lilium longiflorum (Trumpet lily), Chlamydomonas reinhardtii, Barley
宿主
20

3

1
兔
Compatible Secondaries
克隆类型
22

2
多克隆
标记
10

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This ATP6V0D2 antibody is un-conjugated
应用范围
13

13

9

7

3

3

1
Western Blotting (WB), Immunohistochemistry (IHC)

交叉反应（详细）

No cross-reactivity with: mangrove plants,

产品特性

Expected / apparent Molecular Weight of the Antigene: 26 / 31 kDa (Arabidopsis thaliana)

纯化方法

serum

免疫原

KLH-conjugated synthetic peptide chosen from subunit E of plant V-ATPase including Arabidopsis thaliana At4g11150. Peptide is conserved in vacuolar H+-ATPase subunit E, isoform 1 to 3 (VHA-E1).

Top Product

Discover our top product ATP6V0D2 Primary Antibody
anti-ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2) (AA 238-306) antibody
ATP6V0D2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 7A4 unconjugated

anti-ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2) (AA 238-306) antibody
ATP6V0D2 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated

anti-ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2) (AA 251-350) antibody
ATP6V0D2 适用: 人 IF (cc), IF (p), ELISA, IHC (fro), IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

anti-ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2) (AA 251-300) antibody
ATP6V0D2 适用: 人, 大鼠, 小鼠, 兔, 豚鼠, 犬, Cow, Pig WB 宿主: 兔 Polyclonal unconjugated

anti-ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2) (C-Term) antibody
ATP6V0D2 适用: 人, 大鼠, 小鼠, 兔, 豚鼠, 犬, Pig, Bat, 猴 WB 宿主: 兔 Polyclonal unconjugated

anti-ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2) (Middle Region) antibody
ATP6V0D2 适用: 人, 大鼠, 小鼠, 兔, 豚鼠, 犬, Cow, 马, Pig WB 宿主: 兔 Polyclonal unconjugated

anti-ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2) (AA 251-350) antibody (Biotin)
ATP6V0D2 适用: 人 ELISA, IHC (fro), IHC (p) 宿主: 兔 Polyclonal Biotin

anti-ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2) (AA 251-350) antibody (Cy7)
ATP6V0D2 适用: 人 IF (cc), IF (p) 宿主: 兔 Polyclonal Cy7

anti-ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2) (AA 251-350) antibody (HRP)
ATP6V0D2 适用: 人 ELISA, IHC (fro), IHC (p) 宿主: 兔 Polyclonal HRP

anti-ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2) (AA 251-350) antibody (FITC)
ATP6V0D2 适用: 人 IF (cc), IF (p) 宿主: 兔 Polyclonal FITC

应用备注

Recommended Dilution: 1 : 2 000 - 1 : 5000 with alkaline phosphatase (WB), 1 : 50 (IHC).
Cellular [compartment marker] of tonoplast membrane

说明

V-ATPase is very sensitive for the redox of the SDS buffer. We recommend using at least 50-100 mM DTT freshly prepared before handling the sample.Immunostaining protocol using V-ATPase antibodies can be found here.

限制

仅限研究用
状态

Lyophilized

溶解方式

For reconstitution add 10 µL of sterile water.

注意事项

Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

储存条件

-20 °C
Bigeard, Rayapuram, Bonhomme, Hirt, Pflieger: "Proteomic and phosphoproteomic analyses of chromatin-associated proteins from Arabidopsis thaliana." in: Proteomics, Vol. 14, Issue 19, pp. 2141-55, (2014) (PubMed).

Schnell, Han, Miki, Johnson: "Soybean peroxidase propeptides are functional signal peptides and increase the yield of a foreign protein." in: Plant cell reports, (2010) (PubMed).

Hinton, Sennoune, Bond, Fang, Reuveni, Sahagian, Jay, Martinez-Zaguilan, Forgac: "Function of a subunit isoforms of the V-ATPase in pH homeostasis and in vitro invasion of MDA-MB231 human breast cancer cells." in: The Journal of biological chemistry, Vol. 284, Issue 24, pp. 16400-8, (2009) (PubMed).
抗原

ATP6V0D2 (ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2))

别名

V-ATPase (ATP6V0D2 产品)

别名

ATP6D2 antibody, VMA6 antibody, AI324824 antibody, V-ATPase antibody, 1620401A02Rik antibody, ATPase H+ transporting V0 subunit d2 antibody, ATPase, H+ transporting, lysosomal V0 subunit D2 antibody, ATPase H+ transporting V0 subunit D2 antibody, ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d2 L homeolog antibody, ATP6V0D2 antibody, Atp6v0d2 antibody, atp6v0d2.L antibody

背景

AGI Code: At4g11150
Plant vacuole V-ATPase is responsible for energization of transport of ions and metabolites, and acts as well 'house-keeping' and as a stress response enzyme. V-ATPase is a multi-subunit enzyme composed of a membrane sector and a cytosolic catalytic sector. It is related to the FoF1 ATP synthase. Alternative protein names: Vacuolar proton pump subunit E, Protein EMBRYO DEFECTIVE 2448

分子量

expected: 26 kDa, apparent: 31 kDa (Arabidopsis thaliana)

UniProt

Q39258

途径

Transition Metal Ion Homeostasis, Proton Transport