小鼠 anti-人 IgG (Fc Region), (Heavy Chain) Antibody

ABIN302085 产品详细信息, 供应商: Log in to see
抗原
抗原表位
Fc Region, Heavy Chain
5028
2578
1930
1068
1007
857
330
299
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
1
适用

2911
2859
2405
1752
1485
796
623
442
381
380
355
340
307
293
208
148
139
114
41
29
27
25
12
11
9
8
6
5
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
宿主
小鼠
6893
4912
1878
894
703
401
92
54
52
21
9
8
5
5
2
1
1
克隆类型 (克隆位点)
单克隆 ()
应用范围
Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
选项
Supplier
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Supplier Product No.
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免疫原 Fusion protein of human IgG Fc fragment.
克隆位点 EM-07
亚型 IgG1
特异性 The antibody EM-07 reacts with Fc part of human IgG heavy chain and with isolated Fc fragments.
交叉反应 (详细) The antibody EM-07 does not crossreact with IgM.
纯化方法 Purified from hybridoma culture supernatant by protein-A affinity chromatography.
纯度 > 95 % (by SDS-PAGE)
背景 Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75 % of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.
分子量 150 kDa
研究领域 Immunology, Secondary Antibodies
应用备注 Western Blotting:
Application note: The peroxidase conjugate of this antibody is suitable for detection of human IgG Fc fragments by WB.
ELISA:
Application note: The peroxidase conjugate of this antibody is suitable for detection of human IgG Fc fragments by ELISA.

Working concentrations should be determined by the investigator.
限制 仅限研究用
状态 Liquid
浓度 1 mg/mL
缓冲液 Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
储存液 Sodium azide
注意事项 WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
注意事项 Do not freeze.
储存条件 4 °C
储存方法 Store at 2-8 °C. Do not use after expiration date stamped on vial label.
厂商提供的图像
Western Blotting (WB) image for Mouse anti-Human IgG (Fc Region), (Heavy Chain) antibody (ABIN302085) IgG κ light chain (1), IgG λ light chain (2) and IgG Fc fragment (3) purifi...
背景 Brinkmann, Heusser: "T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors." in: Cellular immunology, Vol. 152, Issue 2, pp. 323-32, 1994 (PubMed).

Balogh, Merétey, Falus, Bozsóky: "Serological abnormalities in juvenile chronic arthritis: a review of 46 cases." in: Annals of the rheumatic diseases, Vol. 39, Issue 2, pp. 129-34, 1980 (PubMed).

Franklin: "Structure and function of immunoglobulins." in: Acta endocrinologica. Supplementum, Vol. 194, pp. 77-95, 1975 (PubMed).

Fuller, Keyser: "Serum immunoglobulins after surgical operation." in: Clinical chemistry, Vol. 21, Issue 6, pp. 667-71, 1975 (PubMed).