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Four and A Half LIM Domains 1 (FHL1) 抗体

ABIN2966633 产品详细信息, 供应商: Log in to see
抗原
  • fhl1
  • MGC89165
  • FHL1
  • DKFZp468O1927
  • fhl
  • fhla
  • zgc:92025
  • FHL-1
  • FHL1A
  • FHL1B
  • FLH1A
  • KYOT
  • SLIM
  • SLIM-1
  • SLIM1
  • SLIMMER
  • XMPMA
  • KyoT
  • Fhl1
  • four and a half LIM domains 1
  • four and a half LIM domains 1a
  • Four and a half LIM domains protein 1
  • four and a half lim domains protein 1
  • four and a half LIM domains 1 protein, isoform C
  • fhl1
  • FHL1
  • LOC100357263
  • fhl1a
  • Fhl1
  • FHL1C
适用
人, 小鼠, 大鼠
105
33
26
15
10
10
4
4
4
3
3
2
宿主

41
33
32
克隆类型
多克隆
标记
非结合性
5
3
3
3
3
3
应用范围
Immunochromatography (IC), Immunofluorescence (IF), Western Blotting (WB)
95
57
33
17
16
16
3
2
1
Supplier
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Supplier Product No.
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Available images

'独立验证'标志
抗原 FHL-1
Lot Number AL1F04A
Method validated Immunofluorescence
Positive Control MRL/lpr lupus mice (14 weeks of age)
Negative Control FH knockout MRL/lpr lupus mice
Notes Passed. ABIN2966633 works well on murine kidney samples and shows the expected CFH1 localization.
Primary Antibody ABIN2966633
Secondary Antibody goat anti-rabbit rhodamine conjugated antibody (ThermoFisher Scientific, T-2769, lot 6251)
实验流程
  • Prepare kidneys of wt and complement factor H ko MRL/lpr lupus mice of 14 weeks of age.
  • Snap-freeze kidneys in 2-methylbutane cooled on dry ice and keep at −80°C until use.
  • Sections were cut at 8µm using the cryostat.
  • Dry sections for 30min at RT.
  • Hydrate sections in PBS 2x for 15min at RT.
  • Fix cryostat sections with 4% paraformaldehyde (PFA) for 15min at RT.
  • Wash sections 2x with PBS for 15min at RT.
  • Incubate sections with primary rabbit anti-FHL-1 antibody (antibodies-online, ABIN2966633, AL1F04A) diluted 1:100 in PBS ON at 4°C.
  • Wash sections 2x for 15min with PBS.
  • Incubate sections with secondary goat anti-rabbit rhodamine conjugated antibody (ThermoFisher Scientific, T-2769, lot 6251) diluted 1:100 in PBS for 30min at RT.
  • Wash sections 2x for 15min with PBS.
  • Incubate sections with ActinGreen 488 ReadyProbes Reagent (ThermoFisher Scientific, R37122, lot 1700569) diluted 1:100 in PBS for 30min at RT.
  • Wash sections 2x for 15min with PBS.
  • Image acquisition on a Zeiss microscope (Carl Zeiss, Oberkochen, Germany).
Validation Images
Immunofluorescence validation image for anti-Four and A Half LIM Domains 1 (FHL1) antibody (ABIN2966633) IF staining with ABIN2966633 of kidneys harvested from 14 week old MRL/lpr mice suffi...
免疫原 Recombinant full length protein of human FHL-1
特异性 Recognizes endogenous levels of FHL-1 protein.
产品特性 Rabbit polyclonal antibody to FHL-1
纯化方法 The antibody was purified by immunogen affinity chromatography.
别名 FHL-1 (FHL1 Antibody 摘要)
背景 SLIM1, Four and a half LIM domains protein 1, FHL-1, Skeletal muscle LIM-protein 1, SLIM, SLIM-1
基因ID 2273, 14199, 25177
UniProt Q13642, P97447, Q9WUH4
应用备注 WB (1:500 - 1:2000), IF/IC (1:50 - 1:100)
限制 仅限研究用
状态 Liquid
缓冲液 Liquid in 0.42 % Potassium phosphate, 0.87 % Sodium chloride, pH 7.3, 30 % glycerol, and 0.01 % sodium azide.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 -20 °C
储存方法 Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
有效期 12 months
厂商提供的图像
Immunofluorescence (IF) image for anti-Four and A Half LIM Domains 1 (FHL1) 抗体 (ABIN2966633) Immunofluorescent analysis of FHL-1 staining in Hela cells. Formalin-fixed cells were...
Western Blotting (WB) image for anti-Four and A Half LIM Domains 1 (FHL1) 抗体 (ABIN2966633) Western blot analysis of FHL-1 expression in 22RV1 (A), MCF7 (B), mouse kidney (C) wh...