FHL1 抗体
(1 validation)-
- 抗原 See all FHL1 抗体
- FHL1 (Four and A Half LIM Domains 1 (FHL1))
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This FHL1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC)
- 特异性
- Recognizes endogenous levels of FHL-1 protein.
- 产品特性
- Rabbit polyclonal antibody to FHL-1
- 纯化方法
- The antibody was purified by immunogen affinity chromatography.
- 免疫原
- Recombinant full length protein of human FHL-1
- Top Product
- Discover our top product FHL1 Primary Antibody
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anti-Four and A Half LIM Domains 1 (FHL1) (AA 23-120) antibody
FHL1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2A9 unconjugated
anti-Four and A Half LIM Domains 1 (FHL1) (C-Term) antibodyFHL1 适用: 人, 小鼠, 大鼠, 犬, Cow, 兔, 绵羊, 豚鼠, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Four and A Half LIM Domains 1 (FHL1) (Internal Region) antibodyVerified FHL1 适用: 小鼠, 大鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Four and A Half LIM Domains 1 (FHL1) antibodyFHL1 适用: 人, 大鼠, 犬 WB, IF, FACS 宿主: 小鼠 Monoclonal 2E11 unconjugated
anti-Four and A Half LIM Domains 1 (FHL1) antibodyFHL1 适用: 人, 小鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Four and A Half LIM Domains 1 (FHL1) (AA 51-100) antibodyFHL1 适用: 人, 小鼠, 大鼠, 犬, Cow, Pig, 兔, 绵羊, 豚鼠, Hamster WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Four and A Half LIM Domains 1 (FHL1) (AA 214-240), (C-Term) antibodyFHL1 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB19645 unconjugated
anti-Four and A Half LIM Domains 1 (FHL1) (N-Term) antibodyFHL1 适用: 人, 小鼠, 大鼠, 犬, Cow, 兔, 绵羊, 豚鼠, 马 WB 宿主: 兔 Polyclonal unconjugated
anti-Four and A Half LIM Domains 1 (FHL1) (AA 13-240) antibodyFHL1 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Four and A Half LIM Domains 1 (FHL1) antibodyFHL1 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
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- 应用备注
- WB (1:500 - 1:2000), IF/IC (1:50 - 1:100)
- 限制
- 仅限研究用
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- by
- Alexander Lab, Jacobs School of Medicine and Biomedical Sciences, Department of Medicine, University of Buffalo
- No.
- #101371
- 日期
- 2017.07.19
- 抗原
- FHL-1
- Lot Number
- AL1F04A
- Method validated
- Immunofluorescence
- Positive Control
- MRL/lpr lupus mice (14 weeks of age)
- Negative Control
- FH knockout MRL/lpr lupus mice
- Notes
- Passed. ABIN2966633 works well on murine kidney samples and shows the expected CFH1 localization.
- Primary Antibody
- ABIN2966633
- Secondary Antibody
- goat anti-rabbit rhodamine conjugated antibody (ThermoFisher Scientific, T-2769, lot 6251)
- Full Protocol
- Prepare kidneys of wt and complement factor H ko MRL/lpr lupus mice of 14 weeks of age.
- Snap-freeze kidneys in 2-methylbutane cooled on dry ice and keep at −80°C until use.
- Sections were cut at 8µm using the cryostat.
- Dry sections for 30min at RT.
- Hydrate sections in PBS 2x for 15min at RT.
- Fix cryostat sections with 4% paraformaldehyde (PFA) for 15min at RT.
- Wash sections 2x with PBS for 15min at RT.
- Incubate sections with primary rabbit anti-FHL-1 antibody (antibodies-online, ABIN2966633, AL1F04A) diluted 1:100 in PBS ON at 4°C.
- Wash sections 2x for 15min with PBS.
- Incubate sections with secondary goat anti-rabbit rhodamine conjugated antibody (ThermoFisher Scientific, T-2769, lot 6251) diluted 1:100 in PBS for 30min at RT.
- Wash sections 2x for 15min with PBS.
- Incubate sections with ActinGreen 488 ReadyProbes Reagent (ThermoFisher Scientific, R37122, lot 1700569) diluted 1:100 in PBS for 30min at RT.
- Wash sections 2x for 15min with PBS.
- Image acquisition on a Zeiss microscope (Carl Zeiss, Oberkochen, Germany).
- Experimental Notes
生效 #101371 (Immunofluorescence)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![IF staining with ABIN2966633 of kidneys harvested from 14 week old MRL/lpr mice sufficient and deficient in factor H. Cryosections were fixed, stained and images acquired as described in the protocol.]()
IF staining with ABIN2966633 of kidneys harvested from 14 week old MRL/lpr mice sufficient and deficient in factor H. Cryosections were fixed, stained and images acquired as described in the protocol.
Full Methods -
- 状态
- Liquid
- 缓冲液
- Liquid in 0.42 % Potassium phosphate, 0.87 % Sodium chloride, pH 7.3, 30 % glycerol, and 0.01 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
- 有效期
- 12 months
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- 抗原
- FHL1 (Four and A Half LIM Domains 1 (FHL1))
- 别名
- FHL-1 (FHL1 产品)
- 别名
- fhl1 antibody, MGC89165 antibody, FHL1 antibody, DKFZp468O1927 antibody, fhl antibody, fhla antibody, zgc:92025 antibody, FHL-1 antibody, FHL1A antibody, FHL1B antibody, FLH1A antibody, KYOT antibody, SLIM antibody, SLIM-1 antibody, SLIM1 antibody, SLIMMER antibody, XMPMA antibody, KyoT antibody, Fhl1 antibody, four and a half LIM domains 1 antibody, four and a half LIM domains 1a antibody, Four and a half LIM domains protein 1 antibody, four and a half LIM domains 1 S homeolog antibody, fhl1 antibody, FHL1 antibody, fhl1a antibody, fhl1.S antibody, Fhl1 antibody
- 背景
- SLIM1, Four and a half LIM domains protein 1, FHL-1, Skeletal muscle LIM-protein 1, SLIM, SLIM-1
- 基因ID
- 2273, 14199, 25177
- UniProt
- Q13642, P97447, Q9WUH4
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