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TAF12 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 20kDa (TAF12) (N-Term) 抗体

ABIN2792680 产品详细信息, 供应商: Log in to see
抗原
  • CG17358
  • Dmel\\CG17358
  • T2DA_DROME
  • TAF
  • TAF12
  • TAF30a
  • TAF30alpha
  • TAF30alpha/28
  • TAF30alpha/28/22
  • TAFII30alpha/22
  • TAF[II]30alpha
  • TAF[[II]]
  • TAF[[II]]230
  • TAF[[II]]30alpha
  • TAF[[II]]30alpha/28 TAF[[II]]22
  • TAF[[II]]30alpha/28/22
  • TFIID
  • TFIID 22
  • TFIID 28
  • TFIID-p28
  • Taf22
  • Taf28
  • Taf30alpha
  • Taf32
  • Taf[[II]]30alpha
  • d28/22
  • dTAF12
  • dTAF22
  • dTAF28
  • dTAFII30
  • dTAFII30 alpha
  • dTAF[[II]]30
  • dTAF[[II]]30alpha
  • dTAF[[II]]30alpha/22
  • dmTAF12
  • p22
  • p28
  • 20kDa
  • 2810422D08Rik
  • AW557038
  • Taf2J
  • TAF2J
  • TAFII20
  • taf12-a
  • TBP-associated factor 12
  • TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor
  • TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa
  • Taf12
  • TAF12
  • taf12
抗原表位
N-Term
11
3
2
2
1
1
适用
牛, 犬, 豚鼠, 马, 人, 小鼠, 兔, 大鼠
46
27
25
3
3
3
3
2
2
2
2
2
2
1
1
宿主

34
12
克隆类型
多克隆
标记
非结合性
3
3
2
1
1
1
1
1
1
1
1
1
1
1
应用范围
Chromatin Immunoprecipitation (ChIP), Western Blotting (WB)
27
20
13
5
5
4
1
1
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免疫原 The immunogen is a synthetic peptide directed towards the N terminal region of human TAF12
序列 MNQFGPSALI NLSNFSSIKP EPASTPPQGS MANSTAVVKI PGTPGAGGRL
交叉反应 牛, 犬, 小鸡, 人, 小鼠, 大鼠
预测反应 Cow: 100%, Dog: 100%, Guinea Pig: 92%, Horse: 100%, Human: 100%, Mouse: 85%, Rabbit: 100%, Rat: 92%
产品特性 This is a rabbit polyclonal antibody against TAF12. It was validated on Western Blot using a cell lysate as a positive control.
纯化方法 Affinity Purified
别名 TAF12 (TAF12 Antibody 摘要)
背景 Control of transcription by RNA polymerase II involves the basal transcription machinery which is a collection of proteins. These proteins with RNA polymerase II, assemble into complexes which are modulated by transactivator proteins that bind to cis-regulatory elements located adjacent to the transcription start site. Some modulators interact directly with the basal complex, whereas others may act as bridging proteins linking transactivators to the basal transcription factors. Some of these associated factors are weakly attached while others are tightly associated with TBP in the TFIID complex. Among the latter are the TAF proteins. Different TAFs are predicted to mediate the function of distinct transcriptional activators for a variety of gene promoters and RNA polymerases. TAF12 interacts directly with TBP as well as with TAF2I.Control of transcription by RNA polymerase II involves the basal transcription machinery which is a collection of proteins. These proteins with RNA polymerase II, assemble into complexes which are modulated by transactivator proteins that bind to cis-regulatory elements located adjacent to the transcription start site. Some modulators interact directly with the basal complex, whereas others may act as bridging proteins linking transactivators to the basal transcription factors. Some of these associated factors are weakly attached while others are tightly associated with TBP in the TFIID complex. Among the latter are the TAF proteins. Different TAFs are predicted to mediate the function of distinct transcriptional activators for a variety of gene promoters and RNA polymerases. TAF12 interacts directly with TBP as well as with TAF2I. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: TAF2J, TAFII20
Protein Interaction Partner: PRKAR2B, SUMO1, UBE2I, TAF4B, KAT2B, HIST3H3, SUPT3H, TAF4, ERCC1, TAF10, ELAVL1, CPSF1, TAF3, TBP, TAF1A, TAF1C, ATF7, ATXN7, NFYC, NFYB, NFYA, TAF6, TAF8, TAF9, TAF11, TAF5, TAF15, TAF7, TAF2, TAF1, MYC,
Protein Size: 161
分子量 18 kDa
基因ID 6883
NCBI登录号 NM_005644, NP_005635
UniProt Q16514
研究领域 Chromatin and Nuclear Signaling, Transcription Factors
应用备注 WB Suggested Anti-TAF12 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:312500
Positive Control: Transfected 293T.
Optimal working dilutions should be determined experimentally by the investigator.
说明

Antigen size: 161 AA

限制 仅限研究用
状态 Liquid
浓度 Lot specific
缓冲液 Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freeze-thaw cycles.
储存条件 -20 °C
储存方法 For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
厂商提供的图像
Western Blotting (WB) image for anti-TAF12 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 20kDa (TAF12) (N-Term) 抗体 (ABIN2792680) WB Suggested Anti-TAF12 Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
Chromatin Immunoprecipitation (ChIP) image for anti-TAF12 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 20kDa (TAF12) (N-Term) 抗体 (ABIN2792680) Quiescent human colon carcinoma HCT116 cultures were treated with 10 % FBS for three ...
背景 Denissov, van Driel, Voit, Hekkelman, Hulsen, Hernandez, Grummt, Wehrens, Stunnenberg: "Identification of novel functional TBP-binding sites and general factor repertoires." in: The EMBO journal, Vol. 26, Issue 4, pp. 944-54, 2007 (PubMed).

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