Mediator Complex Subunit 25 (MED25) (N-Term) 抗体

ABIN2784291 产品详细信息, 供应商: Log in to see
抗原
  • Arc92
  • CG12254
  • Dmel\\CG12254
  • LD07688
  • Med25
  • dARC92
  • dDrip97
  • med25
  • ACID1
  • ARC92
  • CMT2B2
  • P78
  • PTOV2
  • RGD1305137
  • 2610034E13Rik
  • 2610529E18Rik
  • ESTM2
  • X83308
  • zgc:63555
  • Mediator complex subunit 25
  • mediator complex subunit 25
  • mediator of RNA polymerase II transcription, subunit 25 homolog (yeast)
  • MED25
  • med25
  • LOC100343629
  • Med25
抗原表位
N-Term
11
10
9
3
1
适用
牛, 犬, 豚鼠, 人, 小鼠, 猪, 大鼠, 斑马鱼
24
16
3
3
3
3
2
1
1
1
1
1
1
1
宿主

24
克隆类型
多克隆
标记
非结合性
2
2
2
2
2
2
应用范围
Chromatin Immunoprecipitation (ChIP), Western Blotting (WB)
23
14
14
4
1
1
1
1
选项
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免疫原 The immunogen is a synthetic peptide directed towards the N terminal region of human MED25
Blocking Peptide Blocking peptide for this product available: ABIN979947
序列 EGLRKHYLLP AIEYFNGGPP AETDFGGDYG GTQYSLVVFN TVDCAPESYV
预测反应 Cow: 100%, Dog: 100%, Guinea Pig: 85%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%, Zebrafish: 100%
产品特性 This is a rabbit polyclonal antibody against MED25. It was validated on Western Blot.
纯化方法 Affinity Purified
别名 MED25 (MED25 Antibody 摘要)
背景 This gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2.
Alias Symbols: ACID1, ARC92, CMT2B2, DKFZp434K0512, MGC70671, P78, PTOV2, TCBAP0758
Protein Interaction Partner: UBC, MED19, MED26, EPAS1, NOTCH1, DREB2A, CTDP1, MED6, THRA, RXRA, RARA, MED1, NR3C1, ESR1, CREBBP, SREBF1, MED25, MED15, MED16, MED13, MED12, MED7, MED17, MED23, MED14, CDK8, ZC3H13, QKI, TRIP4, TADA2A, MED28, OBFC1, TRRAP, UL48, MED9, MED8, HCFC1, MED10
Protein Size: 747
分子量 78 kDa
基因ID 81857
NCBI登录号 NM_030973, NP_112235
UniProt Q71SY5
途径 Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response
应用备注 Optimal working dilutions should be determined experimentally by the investigator.
说明

Antigen size: 747 AA

限制 仅限研究用
状态 Liquid
浓度 Lot specific
缓冲液 Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freeze-thaw cycles.
储存条件 -20 °C
储存方法 For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
厂商提供的图像
Western Blotting (WB) image for anti-Mediator Complex Subunit 25 (MED25) (N-Term) antibody (ABIN2784291) WB Suggested Anti-MED25 Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
Western Blotting (WB) image for anti-Mediator Complex Subunit 25 (MED25) (N-Term) antibody (ABIN2784291) WB Suggested Anti-MED25
Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:.12500 ...
Chromatin Immunoprecipitation (ChIP) image for anti-Mediator Complex Subunit 25 (MED25) (N-Term) antibody (ABIN2784291) Quiescent human colon carcinoma HCT116 cultures were treated with 10 % FBS for three ...
有引用在: Sela, Conkright, Chen, Gilmore, Washburn, Florens, Conaway, Conaway: "Role for human mediator subunit MED25 in recruitment of mediator to promoters by endoplasmic reticulum stress-responsive transcription factor ATF6?." in: The Journal of biological chemistry, Vol. 288, Issue 36, pp. 26179-87, 2013 (PubMed).

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