MADCAM1 抗体
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- 抗原 See all MADCAM1 抗体
- MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This MADCAM1 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 品牌
- BD Pharmingen™
- 产品特性
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The MECA-89 antibody reacts with mucosal vascular addressin MAdCAM-1. In the fetus and neonate, MAdCAM-1 is the predominant vascular addressin on the high endothelial venules (HEV) of peripheral lymph nodes. In adult mice, MAdCAM-1 is preferentially expressed in mucosal lymphoid tissues and lamina propria, it is also expressed on sinus-lining cells in the spleen. MAdCAM-1 expression is upregulated on the HEV of peripheral lymph nodes in adult NOD mice and is involved in the development of diabetes and insulitis. Furthermore, there is evidence that IFN-γ can induce MAdCAM-1 expression in non-mucosal sites in adult mice. MAdCAM-1 is a predominant ligand for integrin α4β7, a lymphocyte mucosal homing receptor, and a facultative ligand for CD62L (L-selectin). MECA-89 mAb binds to the second domain of MAdCAM-1 and does not block MAdCAM-1-dependent binding in vitro. Source of the immunogen was endothelial cells from BALB/c mouse mesenteric and peripheral lymph nodes. Immunohistochemical staining of mouse MadCAM-1: Frozen sections of normal mouse small intestine were reacted with the anti-MadCAM-1 antibody. Cells of the lamina propria and the mucosal lymphoid tissue expressing MadCAM-1 can be identified by the brown labeling of their cell membranes. Amplification 20X.
BD Pharmingen™ Purified Rat Anti-Mouse MAdCAM-1 - Purified - Clone MECA-89 - Isotype Rat IgG2a, κ - Reactivity Ms - 1 mL - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Mouse mesenteric and peripheral lymph node cells
- 克隆位点
- MECA
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product MADCAM1 Primary Antibody
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 31.25 μg/mL
- 缓冲液
- Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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L-selectin-mediated lymphocyte rolling on MAdCAM-1." in: Nature, Vol. 366, Issue 6456, pp. 695-8, (1994) (PubMed).
: "Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1." in: Cell, Vol. 74, Issue 1, pp. 185-95, (1993) (PubMed).
: "A tissue-specific endothelial cell molecule involved in lymphocyte homing." in: Nature, Vol. 331, Issue 6151, pp. 41-6, (1988) (PubMed).
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L-selectin-mediated lymphocyte rolling on MAdCAM-1." in: Nature, Vol. 366, Issue 6456, pp. 695-8, (1994) (PubMed).
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- 抗原
- MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))
- 别名
- MAdCAM-1 (MADCAM1 产品)
- 别名
- MACAM1 antibody, AV211525 antibody, mucosal vascular addressin cell adhesion molecule 1 antibody, MADCAM1 antibody, Madcam1 antibody
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