CAS9 (N-Term) 抗体

ABIN2668367 产品详细信息, 供应商: Log in to see
抗原
抗原表位
N-Term
3
1
适用
Streptococcus pyogenes (S. pyogenes)
8
1
宿主
小鼠
5
4
克隆类型 (克隆位点)
单克隆 ()
标记
非结合性
1
1
应用范围
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
9
4
3
3
3
1
1
选项
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免疫原 This antibody was raised against a recombinant protein within the N-terminal region of Streptococcus pyogene Cas9. This antibody should recognize Cas9 and dCas9 based on the antigen design.
克隆位点 7A9-3A3
亚型 IgG1 kappa
纯化方法 Protein G Chromatography
背景 Cas9 is a nuclease from Streptococcus pyogenes that can be targeted to particular DNA sequences through a guide RNA that results in double-stranded breaks in DNA. Cas9 is part of the CRISPR/Cas9 gene-editing system that can create a DNA break at a specific location with the genome. CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) Probable. In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.
分子量 160 kDa
应用备注 Optimal working dilution should be determined by the investigator.
限制 仅限研究用
浓度 1 μg/μL
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

储存条件 -20 °C
储存方法 Antibodies in solution can be stored at -20 °C for 2 years.
有效期 6 months
厂商提供的图像
Immunofluorescence (IF) image for anti-CAS9 (N-Term) antibody (ABIN2668367) Cas9 antibody (mAb) tested by Immunoflourescence. HeLa cells were transiently transfe...
Western Blotting (WB) image for anti-CAS9 (N-Term) antibody (ABIN2668367) Cas9 antibody (mAb) tested by Immunoprecipitation. 5 µg of Cas9 antibody (mAb) was us...
Western Blotting (WB) image for anti-CAS9 (N-Term) antibody (ABIN2668367) Cas9 antibody (mAb) tested by Western blot. HEK293 cells were transiently transfected...
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