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小鼠 anti-牛 IgG Antibody

ABIN238423 产品详细信息, 供应商: Log in to see
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宿主
小鼠
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克隆类型 (克隆位点)
单克隆 ()
应用范围
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
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免疫原 Fraction of Bovine immunoglobulin.
克隆位点 IVA285-1
亚型 IgG1
交叉反应 (详细) Species reactivity (tested):Bovine.
纯化方法 Protein A affinity chromatography
背景 Synonyms: Bovine Immunoglobulin G
研究领域 Immunology, Secondary Antibodies
应用备注 Western Blotting (Reducing conditions): 0,5-1 μg/mL, overnight in 4C. SDS-PAGE (12 % separating gel). Positive control and sample preparation: BS (bovine serum), dilution 1:50 in Laemmlireducing buffer, boiled in water bath for 3 min. Strongly reacts with bovine IgG light chains, weakly reacts with IgM. Flow Cytometry. Immunohistochemistry (frozen sections).

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
浓度 1.0 mg/mL
缓冲液 PBS, pH 7.4, 15 mM Sodium Azide
储存液 Sodium azide
注意事项 WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
厂商提供的图像
Western Blotting (WB) image for 小鼠 anti-牛 IgG Antibody (ABIN238423) Mouse anti-Cow (Bovine) IgG antibody
背景 Antalíková, Simon, Jankovicová, Horovská, Dusinský, Hluchý: "Monoclonal antibody to the light chain of bovine immunoglobulin." in: Hybridoma (2005), Vol. 25, Issue 5, pp. 309-12, 2006 (PubMed).