SEMA3B 抗体 (AA 100-200)
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- 抗原 See all SEMA3B 抗体
- SEMA3B (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3B (SEMA3B))
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抗原表位
- AA 100-200
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适用
- 人, 小鼠, 大鼠, Cow, 犬, 灵长类
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This SEMA3B antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
- 特异性
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Immunohistochemistry-Paraffin Embedded Sections
Note: SEMA3B protein is secreted and accumulates in the endoplasmic reticulum.
Antigen Unmasking:
Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool
slides on bench-top for 30 minutes.
Staining:
1. Wash sections in deionized water three times for 5 minutes each.
2. Wash sections in wash buffer for 5 minutes.
3. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
4. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4 C.
5. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
6. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
7. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
8. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
9. Wash sections three times in wash buffer for 5 minutes each.
10. Add 100-400 µL DAB substrate to each section and monitor staining closely.
11. As soon as the sections develop, immerse slides in deionized water.
12. Counterstain sections in hematoxylin.
13. Wash sections in deionized water two times for 5 minutes each.
14. Dehydrate sections.
15. Mount coverslips. - 纯化方法
- Aff - Purified
- 免疫原
- A synthetic peptide made to an internal portion of the human SEMA 3B protein sequence (AA100-200).
- 亚型
- IgG
- Top Product
- Discover our top product SEMA3B Primary Antibody
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anti-Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3B (SEMA3B) (AA 100-200) antibody
SEMA3B 适用: 人, 小鼠, 大鼠, Cow, 犬, Hamster WB 宿主: 兔 Polyclonal unconjugated
anti-Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3B (SEMA3B) antibodySEMA3B 适用: 小鼠 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3B (SEMA3B) (AA 570-749) antibodySEMA3B 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3B (SEMA3B) (AA 651-748) antibodySEMA3B 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3B (SEMA3B) (AA 1-635) antibodySEMA3B 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3B (SEMA3B) (AA 569-748) antibodySEMA3B 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3B (SEMA3B) (Middle Region) antibodySEMA3B 适用: 人, 大鼠, Cow, 犬, 豚鼠, 兔 WB 宿主: 兔 Polyclonal unconjugated
anti-Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3B (SEMA3B) (AA 100-200) antibodySEMA3B 适用: 人, 小鼠, 大鼠, Cow, 犬, 猴 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Western Blot 1:5000 , Immunohistochemistry: 1:100 (paraffin-embedded tissue), Immunocytochemistry/Immunofluorescence 1:40 Dilutions listed as a recommendation. Optimal dilution should be determined by investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
- Tris-glycine, 150 mM NaCL and 0.05% Sodium Azide as a preservative.
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 注意事项
- Repeated freeze/thaw cycles
- 储存条件
- -20 °C
- 储存方法
- Store frozen. Aliquot as undiluted antisera and immediately place at -20°C. Antisera may have become trapped in top of vial during shipping. Centrifugation of vial is recommended before opening. Stable for at least 6 months at -20°C. Compromise the integrity of the antiserum.
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- 抗原
- SEMA3B (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3B (SEMA3B))
- 别名
- SEMA3B (SEMA3B 产品)
- 别名
- LUCA-1 antibody, SEMA5 antibody, SEMAA antibody, SemA antibody, semaV antibody, AW208495 antibody, Semaa antibody, sema5 antibody, semaphorin 3B antibody, semaphorin 3B-like antibody, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B antibody, SEMA3B antibody, SEMA3BL antibody, sema3b antibody, Sema3b antibody
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