JE-2A6 binds with high affinity to vitellogenin in whole body homogenate samples from zebrafish. The antibody does not cross-react with vitellogenin from the other cyprinid species carp (Cyprinus carpio), fathead minnow (Pimephales promelas) and roach (Rutilus rutilus).
免疫原
Vitellogenin purified from whole body homogenate of 17ß-estradiol-treated zebrafish.
JE-2A6 binds specifically to zebrafish vitellogenin in both ELISA and western blot. Since assay conditions vary, the optimum dilution should be determined for each particular application. Note: The antibody is dissolved in a buffer containing BSA, and is therefore not recommended to use for coating. Normal dilution range: ELISA: 1:100 - 1:1000 - Western blot: 1:100 ? 1:1000
限制
仅限研究用
状态
Liquid
缓冲液
0.1 M Na-phosphate, 0.15 M NaCl, 1% BSA, 12.3 mM Na-azide, pH 7.4.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.