小鼠 anti-人 Ig (Light Chain), (Chain kappa) Antibody

ABIN120648 产品详细信息, 供应商: Log in to see
抗原
抗原表位
Light Chain, Chain kappa
540
393
289
66
25
16
13
10
7
6
5
3
2
2
1
适用

684
213
74
30
19
15
10
6
5
5
4
4
3
2
1
1
1
1
1
1
1
1
1
1
宿主
小鼠
405
391
158
78
21
7
4
1
1
克隆类型 (克隆位点)
单克隆 ()
应用范围
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
选项
Supplier
Log in to see
Supplier Product No.
Log in to see
免疫原 Crude thymus membrane fraction
克隆位点 MEM-09
亚型 IgG1
交叉反应 (详细) Species reactivity (tested):Human
纯化方法 Affinity Chromatography on Protein A
别名 Immunoglobulin
背景 Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda, both having a molecular weight of 22.5 kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.
研究领域 Secondary Antibodies, Virology
应用备注 Flow Cytometry. Immunoprecipitation. Western Blotting. Immunohistochemistry (paraffin sections). Immunocytochemistry. ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
浓度 1.0 mg/mL
缓冲液 Phosphate buffered saline (PBS) with 15 mM Sodium Azide as preservative, approx. pH 7.4
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C/-20 °C
储存方法 Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.
有效期 12 months
背景 Horejsí, Hilgert, Kristofová, Bazil, Bukovský, Kulhánková et al.: "Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte ..." in: Folia biologica, Vol. 32, Issue 1, pp. 12-25, 1986 (PubMed).